Chanaj-Kaczmarek Justyna, Paczkowska Magdalena, Osmałek Tomasz, Kaproń Barbara, Plech Tomasz, Szymanowska Daria, Karaźniewicz-Łada Marta, Kobus-Cisowska Joanna, Cielecka-Piontek Judyta
Department of Pharmacognosy, Poznan University of Medical Sciences, 4 Swiecickiego Street, 60781 Poznan, Poland.
Department of Pharmaceutical Technology, Poznan University of Medical Sciences, 6 Grunwaldzka Street, 60780 Poznan, Poland.
Pharmaceutics. 2020 Jul 7;12(7):634. doi: 10.3390/pharmaceutics12070634.
is a valued plant material with known anti-inflammatory and antimicrobiological properties. The limitation for its use in the treatment of chronic wounds is the lack of adhesion to the required site of action. Obtaining the lyophilized extract from water-ethanolic extract allowed to prepare valuable material whose biological activity in the wound healing process was confirmed by a positive result of the scratch test. The lyophilized extract was standardized for the contents of the chlorogenic acid and the narcissin. The significant potency of the pharmacological activity has become the reason for studies on its novel applications in combination with the multifunctional chitosan carrier, to create a new, valuable solution in the treatment of chronic wounds. The use of chitosan as a carrier allowed for the controlled release of the chlorogenic acid and the narcissin. These substances, characterized by prolonged release from the chitosan delivery system, were identified as well permeable, based on the results of the studies of the parallel artificial membrane permeability assay (PAMPA Skin) a model simulating permeability through membrane skin. The combination of the lyophilized extract and the chitosan allowed for synergy of action towards hyaluronidase inhibition and effective microbiological activity. Optimization of the hypromellose hydrogel preparation ensuring the required rheological properties necessary for the release of the chlorogenic acid and the narcissin from the chitosan delivery system, as well as demonstrated antimicrobial activity allows indicating formulations of 3% lyophilized extract with chitosan 80/500 in weight ratio 1:1 and 2% or 3% hypromellose as an important support with high compliance of response and effectiveness for patients suffering from chronic wounds.
是一种具有已知抗炎和抗菌特性的珍贵植物材料。其在慢性伤口治疗中的应用限制在于缺乏对所需作用部位的粘附性。从水乙醇提取物中获得冻干提取物，从而制备出了有价值的材料，划痕试验的阳性结果证实了其在伤口愈合过程中的生物活性。冻干提取物针对绿原酸和水仙苷的含量进行了标准化。其显著的药理活性促使人们研究将其与多功能壳聚糖载体结合的新应用，以创造一种治疗慢性伤口的新的有价值的解决方案。使用壳聚糖作为载体可实现绿原酸和水仙苷的控释。基于平行人工膜通透性测定（PAMPA皮肤）模型模拟透过皮肤膜的通透性研究结果，这些从壳聚糖递送系统中具有延长释放特性的物质被确定为具有良好的通透性。冻干提取物与壳聚糖的组合在抑制透明质酸酶和有效微生物活性方面具有协同作用。优化羟丙甲纤维素水凝胶制剂，确保从壳聚糖递送系统中释放绿原酸和水仙苷所需的流变学特性，以及所展示的抗菌活性，表明重量比为1:1的3%冻干提取物与80/500壳聚糖以及2%或3%羟丙甲纤维素的制剂，对于患有慢性伤口的患者而言是重要的支持，具有高度的反应顺应性和有效性。
