Garon D, Krivobok S, Wouessidjewe D, Seigle-Murandi F
Groupe pour l'éude du devenir des xénobiotiques dans l'environnement (GEDEXE), UFR de Pharmacie de Grenoble, Université Joseph Fourier, Meylan, France.
Chemosphere. 2002 Apr;47(3):303-9. doi: 10.1016/s0045-6535(01)00299-5.
Eighteen fungal strains were tested in toxicity assays with surfactants in order to select surfactants and strains tolerant to surfactants for degradation assays. Two nonionic surfactants were used, an alkylphenol ethoxylate, Triton X-100, a sorbitan ester, Tween 80 and an anionic surfactant, sodium dodecyl sulfate. Solubilization and biodegradation tests were conducted in liquid medium batch; fluorene was quantified by HPLC. Results showed the enhancement of fluorene solubilization by the three surfactants, good tolerance of nonionic surfactants by the fungal strains and the enhancement of the biodegradation of fluorene by Doratomyces stemonitis (46-62%) and Penicillium chrysogenum (28-61%) in the presence of Tween 80 (0.324 mM) after 2 days.
为了选择对表面活性剂具有耐受性的表面活性剂和菌株用于降解试验，对18种真菌菌株进行了表面活性剂毒性试验。使用了两种非离子表面活性剂，一种烷基酚聚氧乙烯醚，吐温X-100，一种山梨醇酯，吐温80，以及一种阴离子表面活性剂，十二烷基硫酸钠。在液体培养基批次中进行增溶和生物降解试验；芴通过高效液相色谱法进行定量。结果表明，三种表面活性剂均增强了芴的增溶作用，真菌菌株对非离子表面活性剂具有良好的耐受性，并且在吐温80（0.324 mM）存在的情况下，2天后，茎点霉（46-62%）和产黄青霉（28-61%）对芴的生物降解作用增强。
