Department of Biochemistry, Gulbarga University, Kalaburagi 585106, Karnataka, India.
Department of Biochemistry, Gulbarga University, Kalaburagi 585106, Karnataka, India.
Ecotoxicol Environ Saf. 2018 Apr 30;151:206-211. doi: 10.1016/j.ecoenv.2018.01.019. Epub 2018 Feb 4.
The present investigation was to study the effect of different non-ionic surfactants (Tween-80, Tween-60, Tween-40, Tween-20, Triton X-100) and a rhamnolipid biosurfactant on the degradation of fluorene by Paenibacillus sp. PRNK-6. An enhancement in the growth, as well as fluorene utilization by this strain were observed in the presence of biosurfactant and non-ionic surfactants except Tween-20 and Triton X-100. Triton X-100 and Tween-20 were toxic to this bacterium. The strain PRNK-6 utilized 75% of fluorene (280mg/L) in 24h in an unamended condition. On the other hand, the complete utilization of higher concentration fluorene (320mg/L) by this strain was noticed when the medium was amended with Tween-80 (1.5% v/v) within 24h of incubation. Whereas, 90.6%, 96.5% and 96.7% of fluorene (280mg/L) was utilized when amended with Tween-60 (3.5% v/v), Tween-40 (3% v/v) and biosurfactant (25mg/L) respectively. Biosurfactant promoted the fluorene degradation potential of PRNK-6 as 96.2% of 320mg/L fluorene was degraded within 24h. Further, the added tween series surfactants and a biosurfactant have increased the cell surface hydrophobicity of the PRNK-6. Thus correlating with the enhanced degradation of the fluorene.
本研究旨在研究不同的非离子表面活性剂（吐温-80、吐温-60、吐温-40、吐温-20、Triton X-100）和鼠李糖脂生物表面活性剂对荧光棒降解的影响。在生物表面活性剂和非离子表面活性剂的存在下，观察到该菌株的生长以及荧光棒的利用得到增强，除了吐温-20 和 Triton X-100。Triton X-100 和吐温-20 对该细菌有毒。在未添加条件下，菌株 PRNK-6 在 24 小时内利用了 75%的芴（280mg/L）。另一方面，当在培养基中添加吐温-80（1.5%v/v）时，该菌株在 24 小时的孵育时间内完全利用了较高浓度的芴（320mg/L）。而当添加吐温-60（3.5%v/v）、吐温-40（3%v/v）和生物表面活性剂（25mg/L）时，芴（280mg/L）的利用率分别为 90.6%、96.5%和 96.7%。生物表面活性剂促进了 PRNK-6 降解芴的能力，在 24 小时内降解了 320mg/L 芴的 96.2%。此外，添加的吐温系列表面活性剂和生物表面活性剂增加了 PRNK-6 的细胞表面疏水性。因此，与芴的增强降解相关。
