Chaussain Miller C, Septier D, Bonnefoix M, Lecolle S, Lebreton-Decoster C, Coulomb B, Pellat B, Godeau G
Laboratoire de Physiopathologie des Tissus non Mineralises, Faculté de Chirurgie Dentaire Paris V, Montrouge, France.
Clin Oral Investig. 2002 Mar;6(1):39-50. doi: 10.1007/s00784-001-0143-2.
Free-floating collagen lattice is considered a useful tool for assessing wound healing in vitro. This work compared extracellular matrix remodeling in collagen lattices populated by gingival or dermal fibroblasts. For 21 days we followed gel contraction and changes in cell number of collagen lattices seeded with l.5 x 10(5) fibroblasts of each tissue. We also used indirect immunodetection to study extracellular matrix components, metalloproteinases (MMPs), and their tissues inhibitors (TIMPs). In addition, the presence of MMPs and TIMPs in the culture media was analyzed by zymography and western blotting. No significant difference was found concerning gel contraction and changes in cell number. We observed the early expression of fibrillin I and collagen type III, apparently codistributed and at the end of the gel contraction their disappearance. Concomitantly we demonstrated the expression of MMPs and TIMPs, initially localized in cellular cytoplasm, then spreading in the extracellular compartment, and even found in the culture medium. This remodeling was more rapid and intense with gingival fibroblasts than dermal fibroblasts. In conclusion, gingival fibroblasts seem more efficient at remodeling the connective tissue than dermal fibroblasts and could lead to the better wound healing observed in vivo.
游离胶原晶格被认为是体外评估伤口愈合的一种有用工具。这项工作比较了由牙龈成纤维细胞或真皮成纤维细胞构成的胶原晶格中的细胞外基质重塑情况。在21天的时间里，我们追踪了接种每种组织的1.5×10⁵个成纤维细胞的胶原晶格的凝胶收缩和细胞数量变化。我们还使用间接免疫检测来研究细胞外基质成分、金属蛋白酶（MMPs）及其组织抑制剂（TIMPs）。此外，通过酶谱分析和蛋白质印迹法分析了培养基中MMPs和TIMPs的存在情况。在凝胶收缩和细胞数量变化方面未发现显著差异。我们观察到原纤蛋白I和III型胶原的早期表达，它们明显共分布，并且在凝胶收缩结束时消失。同时，我们证明了MMPs和TIMPs的表达，最初定位于细胞质中，然后扩散到细胞外区室，甚至在培养基中也有发现。这种重塑在牙龈成纤维细胞中比在真皮成纤维细胞中更快且更强烈。总之，牙龈成纤维细胞在重塑结缔组织方面似乎比真皮成纤维细胞更有效，并且可能导致在体内观察到更好的伤口愈合。
