白羽扇豆植物提取物（LU105）对培养的人牙龈成纤维细胞中基质金属蛋白酶（MMP1、MMP2、MMP9）和金属蛋白酶组织抑制剂（TIMP1、TIMP2）产生的影响。

Effects of a vegetable extract from Lupinus albus (LU105) on the production of matrix metalloproteinases (MMP1, MMP2, MMP9) and tissue inhibitor of metalloproteinases (TIMP1, TIMP2) by human gingival fibroblasts in culture.

作者信息

Gaultier F, Foucault-Bertaud A, Lamy E, Ejeil A L, Dridi S M, Piccardi N, Piccirilli A, Msika P, Godeau G, Gogly B

机构信息

Laboratory of Physiopathology of Non-mineralised Tissues, U.F.R. Odontology, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge, France.

出版信息

Clin Oral Investig. 2003 Dec;7(4):198-205. doi: 10.1007/s00784-003-0210-y. Epub 2003 Jun 7.

DOI:10.1007/s00784-003-0210-y

PMID:12802622

Abstract

This study examined the effects of a vegetable extract from Lupinus albus (LU105) on MMPs and TIMPs secreted by human gingival fibroblasts in culture. LU105 was extracted from seeds of L. albus and is freely soluble in water. Gelatin zymography showed that control human gingival fibroblasts maintained in culture for 48 h express pro-MMP2 (progelatinase A) in the culture medium while the active form of MMP2 (gelatinase A), the active form of MMP9 (gelatinase B), and pro-MMP9 (progelatinase B) are not detected. Fibroblasts derived from inflamed gingiva expressed in the culture medium increased amounts of pro-MMP2 (progelatinase A) compared with controls and significant amounts of pro-MMP9 (progelatinase B). LU105 diminished the expression by gingival fibroblasts derived from inflamed tissue of both pro-MMP2 and pro-MMP9. Furthermore LU105 did not modify the amount of TIMP2 expressed in culture by controls or by gingival fibroblasts derived from inflamed tissue. TIMP1 and MMP1 significantly decreased when LU105 was added in the culture media of gingival fibroblasts derived from inflamed tissue compared with control fibroblasts. Thus LU105 seems to offer an opportunity to restore a correct balance between MMP2, MMP9, MMP1, and their natural inhibitors, i.e., TIMP1 and TIMP2 in human inflamed gingiva.

摘要

本研究检测了白羽扇豆（Lupinus albus，LU105）的植物提取物对培养的人牙龈成纤维细胞分泌的基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制因子（TIMPs）的影响。LU105是从白羽扇豆种子中提取的，可自由溶于水。明胶酶谱分析表明，在培养基中培养48小时的对照人牙龈成纤维细胞表达前MMP2（前明胶酶A），而未检测到MMP2的活性形式（明胶酶A）、MMP9的活性形式（明胶酶B）和前MMP9（前明胶酶B）。与对照相比，来自炎症牙龈的成纤维细胞在培养基中表达的前MMP2（前明胶酶A）量增加，且有大量的前MMP9（前明胶酶B）。LU105减少了来自炎症组织的牙龈成纤维细胞对前MMP2和前MMP9的表达。此外，LU105并未改变对照或来自炎症组织的牙龈成纤维细胞在培养物中表达的TIMP2量。与对照成纤维细胞相比，当在来自炎症组织的牙龈成纤维细胞的培养基中添加LU105时，TIMP1和MMP1显著降低。因此，LU105似乎为恢复人炎症牙龈中MMP2、MMP9、MMP1及其天然抑制剂（即TIMP1和TIMP2）之间的正确平衡提供了一个机会。