Sterczała Barbara, Chwiłkowska Agnieszka, Szwedowicz Urszula, Kobielarz Magdalena, Chwiłkowski Bartłomiej, Dominiak Marzena
Dental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland.
Department of Molecular and Cell Biology, Wroclaw Medical University, 50-556 Wroclaw, Poland.
Materials (Basel). 2022 Jan 21;15(3):796. doi: 10.3390/ma15030796.
The present study aimed to compare the action of advanced platelet-rich fibrin (A-PRF+) alone with the action of A-PRF+ combined with autologous gingival fibroblasts. The components released from A-PRF+ conditioned with autogenous fibroblasts that were quantified in the study were fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor-beta1 and 2 (TGFβ1 and TGFβ2), and soluble collagen. A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF at every time point and, after 7 days, significantly higher values of released TGFβ2. A viability test after 72 h showed a significant increase in proliferation fibroblasts after exposition to the factors released from A-PRF+ combined with fibroblasts. Similarly, the degree of wound closure after 48 h was significantly higher for the factors released from A-RRF+ alone and the factors released from A-RRF+ combined with fibroblasts. These results imply that platelet-rich fibrin (PRF) enhanced with fibroblasts can be an alternative method of connective tissue transplantation.
本研究旨在比较单独使用高级富血小板纤维蛋白（A-PRF+）与A-PRF+联合自体牙龈成纤维细胞的作用。在本研究中定量的由自体成纤维细胞调节的A-PRF+释放的成分包括成纤维细胞生长因子（FGF）、血管内皮生长因子（VEGF）、转化生长因子-β1和2（TGFβ1和TGFβ2）以及可溶性胶原蛋白。A-PRF+与成纤维细胞联合在每个时间点均显示出显著更高的VEGF释放值，且在7天后，TGFβ2的释放值显著更高。72小时后的活力测试显示，暴露于A-PRF+与成纤维细胞联合释放的因子后，成纤维细胞增殖显著增加。同样，单独的A-RRF+释放的因子以及A-RRF+与成纤维细胞联合释放的因子在48小时后的伤口闭合程度均显著更高。这些结果表明，用成纤维细胞增强的富血小板纤维蛋白（PRF）可能是一种结缔组织移植的替代方法。
