Cammarano P, Pons S, Londei P
Acta Biol Med Ger. 1975;34(7):1123-35.
The molecular weights and the integrity of the two major components of ribosomal RNA from a wide variety of eukaryotic species, from protozoa to man, has been investigated by polyacrylamide gel electrophoresis under fully denaturing conditions. The results show that the s-rRNA is largely heterogeneous, ranging in size, from 0.65 X 10(6) to 0.96 X 10(6) dalton. The l-rRNA ranges in size from a minimum mol wt of 1.28 X 10(6) to a maximum weight of 1.60--1.66 X 10(6) (of warm-blooded vertebrates, Cephalopoda and Diptera); several intermediate values have been found in lower organisms and Protozoa. The s-rRNA is a truly continous, uninterrupted polynucleotide chain in all groups of organisms (protozoans, plants, fungi and animals). The larger rRNA is a continous un-nicked chain in all of the deuterostomian animals, plants and fungi. However, the l-rRNA of all the protostomian animals and the protozoa is an aggregate molecule consisting of two subunits held together by limited regions of hydrogen bounding; in these organisms the size of the s-rRNA is generally identical to that of the larger fragment of the l-rRNA. Analysis of the molecular weights of the subunits of the l-rRNA in the protostomians and the protozoa suggests that the l-rRNA contains one short stretch, prone to nucleolytic attack, dividing the RNA chain into a molecularly conserved portion (0.65 to 0.72 X 10(6) dalton) and a variable portion (0.65 to 0.96 X 10(6) dalton).
在完全变性条件下，通过聚丙烯酰胺凝胶电泳对从原生动物到人类等多种真核生物核糖体RNA的两种主要成分的分子量和完整性进行了研究。结果表明，小核糖体RNA（s-rRNA）在很大程度上是异质的，大小范围从0.65×10⁶到0.96×10⁶道尔顿。大核糖体RNA（l-rRNA）的大小范围从最小分子量1.28×10⁶到最大分子量1.60 - 1.66×10⁶（温血脊椎动物、头足类动物和双翅目昆虫）；在低等生物和原生动物中发现了几个中间值。在所有生物类群（原生动物、植物、真菌和动物）中，s-rRNA是一条真正连续、不间断的多核苷酸链。在所有后口动物、植物和真菌中，较大的核糖体RNA是一条连续无缺口的链。然而，所有原口动物和原生动物的l-rRNA是一个聚合分子，由通过有限区域的氢键结合在一起的两个亚基组成；在这些生物中，s-rRNA的大小通常与l-rRNA较大片段的大小相同。对原口动物和原生动物中l-rRNA亚基分子量的分析表明，l-rRNA包含一个易于受到核酸酶攻击的短片段，将RNA链分为一个分子保守部分（0.65至0.72×10⁶道尔顿）和一个可变部分（0.65至0.96×10⁶道尔顿）。
