Chen Qi, Peng Wenlie, Xu Anlong
Department of Biochemistry and Center for Biopharmaceutical Research, School of Life Sciences, Zhongshan University, Guangzhou 510275, China.
Biochem Pharmacol. 2002 Apr 15;63(8):1389-96. doi: 10.1016/s0006-2952(02)00871-7.
Acutiaporberine is a novel ether-linked bisalkaloid isolated from the traditional Chinese medicinal herb Thalictrum acutifolium (Hand.-Mazz.) Boivin (TAB). The present study demonstrates for the first time, by means of nuclear staining, DNA agarose gel electrophoresis, and flow cytometry, that acutiaporberine induces apoptosis in PLA-801 cells, a cultured human non-small cell lung cancer (NSCLC) cell line. An immunohistochemical assay and western immunoblot analysis showed down-regulation of the bcl-2 gene and up-regulation of the bax and c-myc genes in the acutiaporberine-treated cells. The observations also indicate that acutiaporberine induces apoptosis of PLA-801 cells in a concentration- and time-dependent manner. These results suggest that acutiaporberine may be a potential, natural apoptosis-inducing agent for NSCLC.
尖刺小檗碱是从传统中药尖叶唐松草（Thalictrum acutifolium (Hand.-Mazz.) Boivin, TAB）中分离得到的一种新型醚键双生物碱。本研究首次通过细胞核染色、DNA琼脂糖凝胶电泳和流式细胞术证明，尖刺小檗碱可诱导人非小细胞肺癌（NSCLC）细胞系PLA-801细胞凋亡。免疫组织化学分析和蛋白质免疫印迹分析显示，经尖刺小檗碱处理的细胞中bcl-2基因下调，bax和c-myc基因上调。这些观察结果还表明，尖刺小檗碱以浓度和时间依赖性方式诱导PLA-801细胞凋亡。这些结果表明，尖刺小檗碱可能是一种潜在的、天然的NSCLC凋亡诱导剂。
