Vandingenen Anick, Hens Korneel, Macours Nathalie, Schoofs Liliane, De Loof Arnold, Huybrechts Roger
Zoological Institute of the KU Leuven, Laboratory for Developmental Physiology and Molecular Biology, Naamsestraat 59, 3000 Leuven, Belgium.
Comp Biochem Physiol B Biochem Mol Biol. 2002 May;132(1):27-35. doi: 10.1016/s1096-4959(01)00529-2.
Angiotensin converting enzyme (ACE) activity, defined as a captopril-inhibitable dipeptidyl carboxypeptidase activity towards 3H-hippurylglycylglycine, was demonstrated in haemolymph, testes and ovaries of the grey fleshfly Neobellieria bullata, hereby suggesting a physiological role for ACE in these particular tissues. While the ACE activity in haemolymph and testes reached relatively high levels, only minute ACE activity could be detected in ovaries throughout the entire vitellogenic cycle. Ovarian extracts of Neobellieria bullata do contain, however, in addition to Neb-TMOF, the Neobellieria bullata trypsin modulating oostatic factor which is an in vitro and a putative in vivo substrate of ACE in circulation, several other heat-stable molecules which individually function either as an ACE substrate or ACE inhibitor. Presumably these ACE interactive factors mask ACE activity in the fly ovaries, as measured by a classic substrate-binding assay. Purification and characterisation of these ACE substrates/inhibitors is in progress and is likely to facilitate the elucidation of the enigmatic physiological relevance of ACE in insects.
血管紧张素转换酶（ACE）活性被定义为对3H-马尿酸酰甘氨酰甘氨酸具有卡托普利抑制作用的二肽基羧肽酶活性，在灰肉蝇（Neobellieria bullata）的血淋巴、睾丸和卵巢中得到证实，这表明ACE在这些特定组织中具有生理作用。虽然血淋巴和睾丸中的ACE活性达到相对较高水平，但在整个卵黄发生周期中，卵巢中仅能检测到微量的ACE活性。然而，除了Neb-TMOF（灰肉蝇胰蛋白酶调节卵发育抑制因子，它是循环中ACE的体外和假定体内底物）外，灰肉蝇的卵巢提取物还含有其他几种热稳定分子，这些分子单独作为ACE底物或ACE抑制剂发挥作用。据推测，通过经典的底物结合试验测量，这些ACE相互作用因子掩盖了果蝇卵巢中的ACE活性。这些ACE底物/抑制剂的纯化和特性鉴定正在进行中，可能有助于阐明ACE在昆虫中神秘的生理相关性。
