Foschi D, Cellerino P, Corsi F, Taidelli T, Morandi E, Rizzi A, Trabucchi E
First Department of Surgery, Institute of Biomedical Sciences Luigi Sacco, Luigi Sacco Hospital, University of Milan, via G.B. Grassi 74, 20157 Milan, Italy.
Surg Endosc. 2002 May;16(5):814-9. doi: 10.1007/s00464-001-9074-x. Epub 2002 Feb 8.
The use of the ultrasonically activated scalpel (UAS) for vessel closure has attained widespread acceptance in many surgical fields. The aim of our study was to investigate the electron microscopic changes to the blood vessels after the application of UAS.
We collected 10 arterial and 10 venous segments from vessels that had previously been closed by UAS during abdominal operations. The samples were then prepared for ultramicroscopic analysis. Pathological changes in the lumen and the three wall layers of the blood vessel were examined under scanning and transmission electron microscopy.
All of the vessel segments showed similar changes: the presence of a blood clot, endothelial cell condensation, coagulative necrosis of the wall, and charring of the vessel at its tip. The edge of the cut vessel were closed by the coagulation bond, which was tied up by collagen fibrils escaped from denaturation.
When ultrasonic energy is applied to tissues, it changes their structure so as to make a new extracellular matrix.
超声刀（UAS）在血管闭合中的应用已在许多外科领域得到广泛认可。我们研究的目的是调查应用UAS后血管的电子显微镜下变化。
我们从腹部手术中先前已用UAS闭合的血管中收集了10个动脉段和10个静脉段。然后将样本制备用于超微分析。在扫描和透射电子显微镜下检查血管腔和三层壁的病理变化。
所有血管段均显示出相似的变化：存在血凝块、内皮细胞凝聚、壁的凝固性坏死以及血管尖端碳化。切断血管的边缘由凝固性结合封闭，该结合被变性逸出胶原蛋白原纤维缠绕。
当超声能量作用于组织时，它会改变其结构以形成新的细胞外基质。
