Takahasi Shoko, Miyahara Kazuro, Ishikawa Hirosi, Ishiguro Naotaka, Suzuki Mitsuyoshi
Veterinary Teaching Hospital, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
J Vet Med Sci. 2002 Mar;64(3):255-9. doi: 10.1292/jvms.64.255.
To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of each dog breed, but no disorder closely associated with mtDNA haplotypes was detected.
为了利用线粒体DNA（mtDNA）单倍型评估患有多种临床疾病的犬类品种的母系效应，从365只犬中扩增出600个碱基对的mtDNA D环区域，并测定其mtDNA序列。600碱基对序列的多样性被分为64种单倍型，包括46种新发现的单倍型，这些单倍型被分为I至IV四个簇。使用mtDNA单倍型进行的谱系分析表明，每个犬类品种在基因上包含一种或几种mtDNA单倍型。当评估遗传背景与临床疾病发生之间的关系时，使用mtDNA单倍型进行的犬类谱系分析显示，这些疾病分布在每个犬类品种的优势mtDNA单倍型中，但未检测到与mtDNA单倍型密切相关的疾病。
