Elde R P, Parsons J A
Am J Anat. 1975 Dec;144(4):541-8. doi: 10.1002/aja.1001440416.
Cell bodies of small to moderate-sized neurons in the female rat hypothalamus were stained specifically for somatostatin (SRIF) by means of the unlabeled antibody-peroxidase-antiperoxidase immunocytochemical method. SRIF-positive perikarya were scattered throughout the periventricular nucleus in a limited region extending from the middle of the optic chiasm to the rostral margin of the median eminence. The same neurons were revealed with either rabbit (R) or guinea pig (GP) anti-SRIF antisera. Positive cell bodies were more readily assessed with GP antibodies because nonspecific background staining was much less with these than with R anti-SRIF. Positive perikarya were not observed in other hypothalamic nuclei and ependymal elements were also immunocytochemically negative.
采用非标记抗体-过氧化物酶-抗过氧化物酶免疫细胞化学方法，对雌性大鼠下丘脑中小等大小神经元的细胞体进行生长抑素（SRIF）特异性染色。SRIF阳性核周体散布于室周核，位于从视交叉中部到正中隆起嘴侧缘的有限区域内。用兔（R）或豚鼠（GP）抗SRIF抗血清均可显示相同的神经元。用GP抗体更容易评估阳性细胞体，因为与R抗SRIF抗体相比，这些抗体的非特异性背景染色要少得多。在其他下丘脑核中未观察到阳性核周体，室管膜成分免疫细胞化学染色也为阴性。
