Kim Jung Il, Hong Seung Bum, Row Kyung Ho
Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, Incheon, South Korea.
J Chromatogr A. 2002 Mar 8;949(1-2):275-80. doi: 10.1016/s0021-9673(01)01273-0.
To isolate epigallocatechin gallate (EGCG) of catechin compounds from Korean green tea (Bosung, Chonnam), a C18 reversed-phase preparative column (250x22 mm) packed with packings of three different sizes (15, 40-63, and 150 microm) was used. The sample extracted with water was partitioned with chloroform and ethyl acetate to remove the impurities including caffeine. The mobile phases in this experiment were composed of 0.1% acetic acid in water, acetonitrile, methanol and ethyl acetate. The injection volume was fixed at 400 microl and the flow rate was increased as the particle size becomes larger. The isolation of EGCG with particle size was compared at a preparative scale and the feasibility of separation of EGCG at larger particle sizes was confirmed. The optimum mobile phase composition for separating EGCG was experimentally obtained at the particle sizes of 15 and 40-63 microm in the isocratic mode, but EGCG was not purely separated at the particle size of 150 microm.
为了从韩国绿茶（宝城，全罗南道）中分离儿茶素化合物表没食子儿茶素没食子酸酯（EGCG），使用了填充有三种不同尺寸（15、40 - 63和150微米）填料的C18反相制备柱（250x22毫米）。用水提取的样品用氯仿和乙酸乙酯进行分配，以去除包括咖啡因在内的杂质。本实验中的流动相由水中0.1%的乙酸、乙腈、甲醇和乙酸乙酯组成。进样体积固定为400微升，流速随着粒径增大而增加。在制备规模下比较了不同粒径对EGCG的分离效果，并证实了较大粒径下EGCG分离的可行性。通过实验得出，在等度模式下，粒径为15和40 - 63微米时，分离EGCG的最佳流动相组成，但在粒径为150微米时，EGCG未能得到纯分离。
