在电动色谱中用十二烷基苯磺酸钠分离多环芳烃。

Separation of polycyclic aromatic hydrocarbons with sodium dodecylbenzenesulfonate in electrokinetic chromatography.

作者信息

Kavran G, Erim F B

机构信息

Technical University of Istanbul, Department of Chemistry, Maslak, Turkey.

出版信息

J Chromatogr A. 2002 Mar 8;949(1-2):301-5. doi: 10.1016/s0021-9673(01)01271-7.

DOI:10.1016/s0021-9673(01)01271-7

PMID:11999747

Abstract

Sodium dodecylbenzenesulfonate (SDBS) was used as an additive in separating a broad range of polycyclic aromatic hydrocarbons (PAHs) by capillary electrophoresis. In the absence of micelles, using an acetonitrile-water (40:60) electrolyte, the separation mechanism was predicted as solvophobic association of the PAH molecules with hydrophobic chains of the SDBS surfactant and a possible pi-pi interaction between aromatic groups of PAHs and SDBS. The effects of voltage, acetonitrile and SDBS concentrations on separation were investigated. SDBS provides a good selectivity for PAHs not only between different ring numbers (1 to 5) but also between the pairs of structural isomers. Under optimum conditions, 11 aromatic compounds were separated with efficiencies between 130,000 and 230,000 theoretical plates. Reproducibilities of migration times range between 1.15 and 1.55% RSD and peak areas between 2 and 9% RSD.

摘要

十二烷基苯磺酸钠（SDBS）被用作添加剂，通过毛细管电泳分离多种多环芳烃（PAHs）。在不存在胶束的情况下，使用乙腈 - 水（40:60）电解质，预测分离机制为PAH分子与SDBS表面活性剂疏水链的疏溶剂缔合以及PAHs芳环基团与SDBS之间可能的π-π相互作用。研究了电压、乙腈和SDBS浓度对分离的影响。SDBS不仅对不同环数（1至5）的PAHs具有良好的选择性，而且对结构异构体对也具有良好的选择性。在最佳条件下，分离出11种芳香族化合物，理论塔板数在130,000至230,000之间。迁移时间的重现性相对标准偏差（RSD）在1.15%至1.55%之间，峰面积的重现性RSD在2%至9%之间。