Cawthon Richard M
Department of Human Genetics, University of Utah, 15 N 2030 E, Room 2100, Salt Lake City, UT 84112, USA.
Nucleic Acids Res. 2002 May 15;30(10):e47. doi: 10.1093/nar/30.10.e47.
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.
长期以来,人们一直认为,使用设计用于与TTAGGG和CCCTAA重复序列杂交的寡核苷酸引物通过PCR扩增来测量脊椎动物DNA中的端粒是不可能的,因为预期只会产生引物二聚体衍生的产物。在此,我们提出了一对引物,它消除了这个问题,使得在封闭管中基于荧光的检测能够简单快速地测量端粒。这种检测方法将有助于对端粒生物学及其在疾病和衰老的分子病理生理学中所起作用的研究。