Maibach H I, Leaffer M A, Skinner W A
Arch Dermatol. 1975 Nov;111(11):1444-5.
We assess the percutaneous penetration potential of two commercially distributed semipermanent hair dyes. Utilizing several solvent systems in conjunction with thin-layer chromatography (TLC), we found that the commercial formulations contained at least nine component chemicals. Eight adult volunteers had their hair dyed with these preparations and 24-hour urine specimens were collected daily for five days. The urine was lyophilized and extracted with diethyl ether under acid and alkaline conditions. The TLC of urine from four of the eight subjects, taken from one to four days after dyeing the hair, disclosed a purple spot. This spot, though not identified, was the same with both dye preparations. It is likely that these hair dyes not uncommonly penetrate the skin.
我们评估了两种商业销售的半永久性染发剂的经皮渗透潜力。结合使用几种溶剂系统和薄层色谱法（TLC），我们发现商业配方中至少含有九种化学成分。八名成年志愿者用这些制剂染发，并连续五天每天收集24小时尿液样本。尿液经冻干处理后，在酸性和碱性条件下用乙醚萃取。对八名受试者中四名在染发后1至4天采集的尿液进行薄层色谱分析，发现有一个紫色斑点。这个斑点虽然未被鉴定，但两种染发制剂的斑点相同。这些染发剂很可能经常会渗透皮肤。
