Shaish A, Keren G, Chouraqui P, Levkovitz H, Harats D
Institute of Lipid and Atherosclerosis Research, Tel-Hashomer, Israel.
Pathobiology. 2001;69(4):225-9. doi: 10.1159/000055947.
The aim of the present study was to compare the accumulation of (125)I-labeled low-density lipoproteins (LDL), oxidized LDL (oxLDL), HDL and BSA in advanced atherosclerotic lesions of apoE-deficient mice.
(125)I-lipoproteins or (125)I-BSA were injected into the tail vein of apoE-deficient mice. Blood clearance of (125)I-lipoproteins and (125)I-BSA and their accumulation in atherosclerotic lesions were assayed.
Blood clearance of (125)I-LDL and (125)I-HDL was moderate, and approximately 30% of the injected lipoproteins were present in plasma 24 h following injection. oxLDL was removed much faster from plasma, and less than 10% of (125)I-oxLDL was present in the circulation 30 min after (125)I-oxLDL injection. The clearance of (125)I-BSA from the circulation was slower than the lipoprotein clearance. The highest accumulation of LDL, oxLDL, HDL and BSA was detected in atherosclerotic lesions in the aortic arch and abdominal aorta, while lower accumulation was detected in the less atherosclerotic descending thoracic aorta.
Our findings demonstrate that both (125)I-HDL and (125)I-BSA as well as (125)I-LDL are accumulated in atherosclerotic plaques and that they can be used for the detection of atherosclerotic lesions.
本研究旨在比较载脂蛋白E缺乏小鼠晚期动脉粥样硬化病变中¹²⁵I标记的低密度脂蛋白（LDL）、氧化型LDL（oxLDL）、高密度脂蛋白（HDL）和牛血清白蛋白（BSA）的蓄积情况。
将¹²⁵I标记的脂蛋白或¹²⁵I标记的BSA注入载脂蛋白E缺乏小鼠的尾静脉。检测¹²⁵I标记的脂蛋白和¹²⁵I标记的BSA的血液清除率及其在动脉粥样硬化病变中的蓄积情况。
¹²⁵I-LDL和¹²⁵I-HDL的血液清除率适中，注射后24小时约30%的注入脂蛋白存在于血浆中。oxLDL从血浆中清除得更快，¹²⁵I-oxLDL注射后30分钟，循环中¹²⁵I-oxLDL的含量不到10%。¹²⁵I-BSA从循环中的清除比脂蛋白清除慢。在主动脉弓和腹主动脉的动脉粥样硬化病变中检测到LDL、oxLDL、HDL和BSA的最高蓄积，而在动脉粥样硬化程度较轻的胸降主动脉中检测到的蓄积较低。
我们的研究结果表明，¹²⁵I-HDL、¹²⁵I-BSA以及¹²⁵I-LDL都在动脉粥样硬化斑块中蓄积，并且它们可用于检测动脉粥样硬化病变。
