Tuovinen O H, Kelley B C, Nicholas D J
Arch Microbiol. 1975 Oct 27;105(2):123-7. doi: 10.1007/BF00447125.
Sulphate was rapidly bound by cell suspensions of Thiobacillus ferrooxidans. The binding was depressed by tetrathionate but was unaffected by Group VI anions, cysteine or methionine. Increasing uptake of sulphate was observed in cell suspensions incubated in the presence of ferrous iron. The bulk of 35S-sulphate was removed from the organisms by washing with dilute sulphuric acid and the remaining label was incorporated into cold trichloroacetic acid-soluble compounds. 35S-labelled adenosine 5'-sulphatophosphate was produced from ATP and 35S-sulphate by cell suspensions and in cell-free extracts. There was no evidence for the production of adenosine 3'-phosphate 5'-sulphatophosphate assayed by a very sensitive bioluminescence method.
硫酸根能迅速被氧化亚铁硫杆菌的细胞悬液结合。这种结合作用受到连四硫酸盐的抑制，但不受第Ⅵ族阴离子、半胱氨酸或蛋氨酸的影响。在含有亚铁离子的情况下孵育细胞悬液时，观察到硫酸根的摄取量增加。通过用稀硫酸洗涤，大部分的35S-硫酸根从生物体中去除，剩余的放射性标记物被整合到冷的三氯乙酸可溶化合物中。细胞悬液和无细胞提取物能利用ATP和35S-硫酸根产生35S-标记的腺苷5'-磷酸硫酸酯。通过一种非常灵敏的生物发光方法检测，没有证据表明能产生腺苷3'-磷酸5'-硫酸酯。
