大肠杆菌K-12中负责硫酸盐、硒酸盐和亚硒酸盐转运的单一转运蛋白。
Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12.
作者信息
Lindblow-Kull C, Kull F J, Shrift A
出版信息
J Bacteriol. 1985 Sep;163(3):1267-9. doi: 10.1128/jb.163.3.1267-1269.1985.
Abstract
A Michaelis-Menten kinetic analysis of the transport of sulfate, selenate, and selenite into Escherichia coli K-12 showed that the three dianions were transported by the same carrier. Km values, used as a measure of the affinity of each ligand for the carrier, showed that sulfate was bound 5 times more tightly than selenate and 37 times more tightly than selenite. The specificity ratio, Vmax/Km, also indicated that sulfate was the preferred ligand. There was little difference in the ratios for selenate and selenite.
摘要
对硫酸盐、硒酸盐和亚硒酸盐转运到大肠杆菌K-12中的米氏动力学分析表明，这三种二价阴离子是由同一载体转运的。用作衡量每个配体与载体亲和力的米氏常数（Km值）表明，硫酸盐与载体的结合紧密程度是硒酸盐的5倍，是亚硒酸盐的37倍。特异性比率Vmax/Km也表明硫酸盐是首选配体。硒酸盐和亚硒酸盐的该比率差异不大。
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