Petinaki E, Miriagou V, Tzouvelekis L S, Hatzi F, Legakis N J, Maniatis A N
Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece.
Diagn Microbiol Infect Dis. 2002 Apr;42(4):279-82. doi: 10.1016/s0732-8893(01)00362-5.
Oxacillin resistance was examined in 258 coagulase-negative staphylococci from Greek hospitals. mecA DNA was detected in 168 isolates, which were also resistant to oxacillin by agar dilution and disk diffusion, according to the current NCCLS breakpoints. Both methods exhibited a relatively low specificity misclassifying 21 and 19 of the 90 mecA-negative isolates respectively as oxacillin resistant. In contrast, an anti-PBP 2a latex agglutination test, applied after induction by oxacillin, correctly classified 163 mecA-positive (sensitivity 97%) and 88 mecA-negative isolates (specificity 97.7%).
对来自希腊医院的258株凝固酶阴性葡萄球菌进行了苯唑西林耐药性检测。根据当前美国国家临床实验室标准化委员会（NCCLS）的断点标准，在168株分离菌中检测到mecA基因，这些分离菌通过琼脂稀释法和纸片扩散法对苯唑西林也耐药。两种方法均表现出相对较低的特异性，分别将90株mecA基因阴性分离菌中的21株和19株误分类为苯唑西林耐药。相比之下，在经苯唑西林诱导后进行的抗PBP 2a乳胶凝集试验，正确分类了163株mecA基因阳性分离菌（敏感性97%）和88株mecA基因阴性分离菌（特异性97.7%）。
