Woods Wendi, Ramotar Karam, Lem Paul, Toye Baldwin
Department of Pathology and Laboratory Medicine, The Ottawa Hospital, Ottawa, Ontario K1H 8L6, Canada.
Diagn Microbiol Infect Dis. 2002 Apr;42(4):291-4. doi: 10.1016/s0732-8893(02)00365-6.
One hundred and ninety-three isolates of coagulase-negative staphylococci (CoNS) were tested against oxacillin by agar dilution, disk diffusion, and Vitek (GPS-105 card), and the presence of the mecA gene determined by multiplex PCR. The results obtained by all testing methods were in agreement for 190 isolates. Two mecA-negative isolates (S. lugdunensis and S. haemolyticus) had MICs of < or = 0.25 microg/ml by agar dilution and Vitek but were resistant by disk diffusion. One mecA-positive isolate was resistant by Vitek and disk diffusion but had an agar dilution MIC of < or = 0.25 microg/ml. For the species of CoNS tested, oxacillin susceptibility results obtained with the Vitek GPS-105 card and disk diffusion correlated well with results obtained by National Committee for Clinical Laboratory Standards agar dilution and with the presence of the mecA gene.
采用琼脂稀释法、纸片扩散法和Vitek（GPS-105卡）对193株凝固酶阴性葡萄球菌（CoNS）进行了苯唑西林药敏试验，并用多重PCR法检测mecA基因的存在情况。所有检测方法对190株菌的检测结果一致。两株mecA基因阴性菌株（路邓葡萄球菌和溶血葡萄球菌）经琼脂稀释法和Vitek检测，苯唑西林的最低抑菌浓度（MIC）≤0.25μg/ml，但经纸片扩散法检测为耐药。一株mecA基因阳性菌株经Vitek和纸片扩散法检测为耐药，但琼脂稀释法测得的MIC≤0.25μg/ml。对于所检测的CoNS菌种，Vitek GPS-105卡和纸片扩散法所得的苯唑西林药敏结果与美国国家临床实验室标准委员会琼脂稀释法所得结果以及mecA基因的存在情况相关性良好。
