Wohlrab F, Essbach H, Krautschick I
Acta Histochem. 1979;65(2):258-68.
We have investigated the localization and demonstration of sorbitol dehydrogenase activity in the rat kidney by comparative histochemical-electrophoretical technique. Under histochemical conditions an adequate demonstration of the activity of the sorbitol dehydrogenase in native sections is possible by membrane incubating technique in presence of PMS and KCN. In the glomerulum the strong and uniform reaction with regard to the specifity is not clear. In the glomerula of semithin sections from glutaraldehyde fixed, incubated and Epon-embedded cryostate sections the reaction product is localized predominantly in the maesangium cells. By micropolyacrylamidgel gradient electrophoresis of the supernatant from the kidney cortex and of isolated glomerula 2 sorbitol dehydrogenase fractions were found. The results are discussed in connection with the specifity of the reaction.
我们采用比较组织化学 - 电泳技术研究了大鼠肾脏中山梨醇脱氢酶活性的定位与显示。在组织化学条件下，通过在存在吩嗪硫酸甲酯（PMS）和氰化钾（KCN）的情况下进行膜孵育技术，能够在天然切片中充分显示山梨醇脱氢酶的活性。在肾小球中，关于特异性的强烈且均匀的反应并不清晰。在经戊二醛固定、孵育并包埋于环氧树脂的半薄切片的肾小球中，反应产物主要定位于系膜细胞。通过对肾皮质上清液和分离出的肾小球进行微量聚丙烯酰胺凝胶梯度电泳，发现了2个山梨醇脱氢酶组分。结合反应的特异性对结果进行了讨论。
