[山梨醇对体内山梨醇脱氢酶活性的影响]

[Influence in vivo of sorbitol on sorbitol dehydrogenase activity].

作者信息

Treves C, Casey H, Bianchi A, Firenzuoli A M, Zanobini A

出版信息

Boll Soc Ital Biol Sper. 1979 Nov 30;55(22):2387-90.

Abstract

Previous researches carried out on the metabolism of sorbitol in the rat showed the influence of this polyalcohol on the activity of some dehydrogenases NAD and NADP-dependent and on lacate/pyruvate, NADH/NAD and NADPH/NADP ratios. Since sorbitol dehydrogenase (SDH, EC 1.1.1.14) is an enzyme with a great affinity for sorbitol, it seemed interesting to investigate the effect of a sorbitol-enriched diet on SDH activity in the rat liver after different periods of dietary treatment (20, 40, 60 days). SDH was assayed by a continuous optical test according to Bücher et al. The data obtained show a repressive action on SDH activity by a sorbitol-enriched diet. In fact, whilst after 20 days of treatment SDH activity decreased of about 18% in respect of the controls, after 60 days a decrease of 80% was observed. This result is so interesting to stimulate other investigations in vitro on the kinetics of such metabolic reaction in the attempt to explain the relation between the enzyme and its substrate.

摘要

先前对大鼠山梨醇代谢的研究表明，这种多元醇对一些依赖NAD和NADP的脱氢酶的活性以及乳酸/丙酮酸、NADH/NAD和NADPH/NADP比率有影响。由于山梨醇脱氢酶（SDH，EC 1.1.1.14）是一种对山梨醇具有高度亲和力的酶，因此研究在不同饮食处理期（20、40、60天）后，富含山梨醇的饮食对大鼠肝脏中SDH活性的影响似乎很有意思。根据比歇尔等人的方法，通过连续光学测试对SDH进行测定。获得的数据表明，富含山梨醇的饮食对SDH活性具有抑制作用。事实上，在处理20天后，SDH活性相对于对照组降低了约18%，而在60天后，观察到降低了80%。这一结果非常有趣，足以激发其他关于这种代谢反应动力学的体外研究，以试图解释该酶与其底物之间的关系。