Wohlrab F, Feustel A
Acta Histochem. 1980;67(2):234-46.
Using PGF2 alpha as substrate we have investigated the demonstration and localization of NAD- and NADP-15-hydroxyprostaglandin dehydrogenase in the kidney of developing and adult rat kidney. Under histochemical conditions an adequate demonstration of the soluble PGDH in native sections is possibly by membrane incubating technic in presence of specific coenzymes. The effectors PMS and KCN showed a decreasing effect on the enzyme activity. In the developing and adult rat kidney the activity of NAD-PGDH was localized predominantly to medullary rays and inner cortex. We have found the reaction product in the following kidney substructures: Pars recta tubuli, distal convoluted tubule, ascending limb of Henle, collecting tubule and lower in proximal convoluted tubule and in the glomerular cells. The NADP-PGDH was localized only in the cortex structures, but the reaction was uneven and the localization of the reaction product was diffuse. The results are discussed in connection with the specificity of the reaction.
以前列腺素F2α为底物，我们研究了NAD - 和NADP - 15 - 羟基前列腺素脱氢酶在发育中和成年大鼠肾脏中的显示及定位。在组织化学条件下，通过在特定辅酶存在下的膜孵育技术，有可能在天然切片中充分显示可溶性前列腺素脱氢酶。效应物PMS和KCN对酶活性有降低作用。在发育中和成年大鼠肾脏中，NAD - 前列腺素脱氢酶的活性主要定位于髓放线和内皮质。我们在以下肾脏亚结构中发现了反应产物：近端小管直部、远曲小管、髓袢升支、集合小管，以及近端小管较低部位和肾小球细胞。NADP - 前列腺素脱氢酶仅定位于皮质结构，但反应不均匀，反应产物的定位弥散。结合反应的特异性对结果进行了讨论。
