Bremner I, Davies N T
Biochem J. 1975 Sep;149(3):733-8. doi: 10.1042/bj1490733.
The isolation of two forms of hepatic zinc-thioneins after either zinc injection into rats or partial restriction of their food intake is described. The proteins differed slightly in their amino acid composition and electrophoretic mobilities. Increases in liver zinc content after both treatments were synchronous with, and associated almost completely with, increased zinc-binding to these proteins. The time-course for the appearance and disappearance of the zinc proteins is shown. It is suggested that metallothionein is involved in the normal metabolism of zinc, perhaps in some temporary storage or detoxication capacity.
本文描述了在给大鼠注射锌或部分限制其食物摄入量后,分离出两种形式的肝脏锌硫蛋白的过程。这两种蛋白质在氨基酸组成和电泳迁移率上略有不同。两种处理后肝脏锌含量的增加与这些蛋白质锌结合能力的增加同步,且几乎完全相关。文中展示了锌蛋白出现和消失的时间进程。研究表明,金属硫蛋白参与锌的正常代谢,可能具有某种临时储存或解毒功能。