从注射铜的大鼠肝脏中分离（铜、锌）-硫蛋白。
Isolation of (copper, zinc)-thioneins from the livers of copper-injected rats.
作者信息
Bremner I, Young B W
出版信息
Biochem J. 1976 Aug 1;157(2):517-20. doi: 10.1042/bj1570517.
Abstract
The mixed copper- and zinc-binding proteins, with mol.wt. of approx. 12000, induced in rat liver after injection of copper were isolated and characterized as metallothioneins. Three separate forms were obtained, with 7-11% total metal in the protein, equivalent to 2.6-1.6 cysteine residues/metal atom.
摘要
注射铜后在大鼠肝脏中诱导产生的分子量约为12000的铜锌结合混合蛋白被分离出来，并被鉴定为金属硫蛋白。得到了三种不同的形式，蛋白质中总金属含量为7 - 11%，相当于每金属原子有2.6 - 1.6个半胱氨酸残基。
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本文引用的文献
1
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Biochem J. 1962 Dec;85(3):480-5. doi: 10.1042/bj0850480.
2
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Biochem J. 1972 Feb;126(3):491-8. doi: 10.1042/bj1260491.
3
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FEBS Lett. 1974 Feb 15;39(2):229-34. doi: 10.1016/0014-5793(74)80057-8.
4
The effect of copper loading on the distribution of copper in rat liver cytosol.
Biochem Med. 1973 Aug;8(1):78-91. doi: 10.1016/0006-2944(73)90011-2.
5
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Q Rev Biophys. 1974 Feb;7(1):75-124. doi: 10.1017/s0033583500001359.
6
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