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大鼠肝细胞溶胶中硫醇转移酶催化的蛋白质混合二硫键中二硫键的还原。

Reduction of disulphide bonds in proteins mixed disulphides catalysed by a thioltransferase in rat liver cytosol.

作者信息

Mannervik B, Axelsson K

出版信息

Biochem J. 1975 Sep;149(3):785-8. doi: 10.1042/bj1490785.

DOI:10.1042/bj1490785
PMID:1201003
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165689/
Abstract

The reduction of mixed disulphides of some proteins and GSH [Protein(-SSG)n] is accomplished with GSH as a reductant and a thioltransferase from rat liver as a catalyst, thus: See article. The spontaneous reaction is negligible in comparison with the enzymic reaction in vivo, and any direct reduction with glutathione reductase is not detectable with the substrates used. The reduction is only indirectly dependent on NADPH, which is required for the regeneration of GSH from GSSG. Other protein disulphides apparently are reduced via analogous GSH-dependent reactions

摘要

某些蛋白质与谷胱甘肽的混合二硫化物[蛋白质(-SSG)n]的还原反应，是以谷胱甘肽作为还原剂、大鼠肝脏中的硫醇转移酶作为催化剂来完成的，具体如下：见文章。与体内的酶促反应相比，自发反应可忽略不计，并且在所使用的底物中，无法检测到谷胱甘肽还原酶的任何直接还原作用。该还原反应仅间接依赖于NADPH，NADPH是将GSSG再生为GSH所必需的。其他蛋白质二硫化物显然也是通过类似的谷胱甘肽依赖性反应被还原的

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Reduction of disulphide bonds in proteins mixed disulphides catalysed by a thioltransferase in rat liver cytosol.大鼠肝细胞溶胶中硫醇转移酶催化的蛋白质混合二硫键中二硫键的还原。
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2
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本文引用的文献

1
Synthesis and characterization of sodium cysteine-S-sulfate monohydrate.半胱氨酸 - S - 硫酸盐一水合钠的合成与表征
Anal Biochem. 1963 Apr;5:330-7. doi: 10.1016/0003-2697(63)90085-x.
2
The reduction of the L-cysteine-glutathione mixed disulfide in rat liver. involvement of an enzyme catalyzing thiol-disulfide interchange.大鼠肝脏中L-半胱氨酸-谷胱甘肽混合二硫化物的还原。一种催化硫醇-二硫化物交换的酶的作用。
FEBS Lett. 1970 Mar 16;7(1):26-28. doi: 10.1016/0014-5793(70)80608-1.
3
The preparation, properties, and reactivation of the mixed disulfide derivative of egg white lysozyme and L-cystine.蛋清溶菌酶与L-胱氨酸混合二硫键衍生物的制备、性质及再活化
J Biol Chem. 1967 Sep 10;242(17):3789-98.
4
Cellular mixed disulphides between thiols and proteins, and their possible implication for radiation protection.硫醇与蛋白质之间的细胞混合二硫化物及其对辐射防护的潜在影响。
Biochem Pharmacol. 1968 Feb;17(2):177-86. doi: 10.1016/0006-2952(68)90321-3.
5
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Biochem J. 1970 Sep;119(3):353-7. doi: 10.1042/bj1190353.
6
Free and protein-bound glutathione in normal and cataractous human lenses.正常及白内障患者晶状体中游离型和蛋白结合型谷胱甘肽
Biochem J. 1970 May;117(5):957-60. doi: 10.1042/bj1170957.
7
Mechanism of action of enzymes catalyzing thiol-disulfide interchange. Thioltransferases rather than transhydrogenases.催化硫醇-二硫键交换的酶的作用机制。是硫醇转移酶而非转氢酶。
FEBS Lett. 1974 Jan 15;38(3):263-7. doi: 10.1016/0014-5793(74)80068-2.
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Biochemistry. 1973 Sep 11;12(19):3667-72. doi: 10.1021/bi00743a015.
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The occurrence of protein-bound mixed disulfides in rat tissues.大鼠组织中蛋白质结合混合二硫化物的出现。
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FEBS Lett. 1974 Mar 1;39(3):296-300. doi: 10.1016/0014-5793(74)80134-1.