中间体在线粒体脂肪酸氧化中的作用。
The role of intermediates in mitochondrial fatty acid oxidation.
作者信息
Stanley K K, Tubbs P K
出版信息
Biochem J. 1975 Jul;150(1):77-88. doi: 10.1042/bj1500077.
Abstract
- Rat liver mitochondria oxidizing [16-14C]palmitoylcarnitine accumulate saturated long-chain thiester intermediates which may be detected by radio-g.1.c.2. Time-courses of intermediate accumulation display no product-precursor relationships and the end product, measured as [14C]citrate, is produced without a detectable initial lag. 3. A short pulse of [16-14C]palmitoylcarnitine followed by unlabelled palmitoylcarnitine showed that the observed intermediates(at least in the greater part)were not the direct precursors of [14C]citrate. 4. The quantity of saturated intermediates depended on the total accumulated flux of acyl units through the pathway provided that some mitochondrial CoA and unused substrate remained. 5. In the presence of rotenone and carnitine, 2-unsaturated, 3-unsaturated and 3-hydroxy intermediates were formed as well as saturated intermediates...
摘要
氧化[16 - ¹⁴C]棕榈酰肉碱的大鼠肝脏线粒体积累饱和长链硫酯中间体，这些中间体可通过放射性气相色谱法检测到。
中间体积累的时间进程未显示出产物 - 前体关系，以[¹⁴C]柠檬酸测量的终产物在没有可检测到的初始延迟的情况下产生。
用[16 - ¹⁴C]棕榈酰肉碱进行短脉冲处理，随后加入未标记的棕榈酰肉碱，结果表明观察到的中间体（至少大部分）不是[¹⁴C]柠檬酸的直接前体。
饱和中间体的量取决于通过该途径的酰基单位的总积累通量，前提是仍有一些线粒体辅酶A和未使用的底物。
在鱼藤酮和肉碱存在的情况下，除了饱和中间体之外，还形成了2 - 不饱和、3 - 不饱和和3 - 羟基中间体……
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本文引用的文献
1
Steady-state concentrations of coenzyme A, acetyl-coenzyme A and long-chain fatty acyl-coenzyme A in rat-liver mitochondria oxidizing palmitate.在氧化棕榈酸酯的大鼠肝脏线粒体中辅酶A、乙酰辅酶A和长链脂肪酰辅酶A的稳态浓度。
Biochem J. 1965 Nov;97(2):587-94. doi: 10.1042/bj0970587.
2
An Extra-Mitochondrial Enzyme System from Peanuts Catalyzing the beta-Oxidation of Fatty Acids.一种来自花生的催化脂肪酸β-氧化的线粒体外酶系统。
Plant Physiol. 1964 Nov;39(6):932-8. doi: 10.1104/pp.39.6.932.
3
Relative rates of conversion of the various carbon atoms of palmitic acid to carbon dioxide by the intact rat.完整大鼠体内棕榈酸的各种碳原子转化为二氧化碳的相对速率。
J Biol Chem. 1950 Jun;184(2):735-44.
4
Biosynthesis of saturated fatty acids.饱和脂肪酸的生物合成。
Fed Proc. 1961 Dec;20:941-51.
5
CHAIN-SHORTENING OF STEARIC ACID IN THE INTACT RAT.完整大鼠体内硬脂酸的链缩短
Biochim Biophys Acta. 1965 Feb 1;98:36-40. doi: 10.1016/0005-2760(65)90006-8.
6
7
8
METABOLISM OF PALMITIC ACID IN PERFUSED RAT HEART.灌注大鼠心脏中棕榈酸的代谢
Am J Physiol. 1963 Oct;205:766-70. doi: 10.1152/ajplegacy.1963.205.4.766.
9
10
Acetoacetate formation in liver. III. On the mechanism of acetoacetate formation from palmitic acid.肝脏中乙酰乙酸的形成。III. 关于由棕榈酸形成乙酰乙酸的机制。
J Biol Chem. 1954 Aug;209(2):537-48.
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