Schliephake Henning, Scharnweber Dieter, Dard Michael, Rössler Sophie, Sewing Andreas, Meyer Jörg, Hoogestraat Dennis
Department of Oral and Maxillofacial Surgery, University Medical School, Hannover, Germany.
Clin Oral Implants Res. 2002 Jun;13(3):312-9. doi: 10.1034/j.1600-0501.2002.130312.x.
The aim of the present study was to analyse the effect of organic coating of titanium implants on periimplant bone formation and bone/implant contact. Three types of implants were used: (i) Ti6Al4V implants with polished surface (control 1) (ii) Ti6Al4V implants with collagen coating (control 2) (iii) Ti6Al4V implants with collagen coating and covalently bound RGD peptides. All implants had square cross-sections with an oblique diameter of 4.6 mm and were inserted press fit into trephine burr holes of 4.6 mm in the mandibles of 10 beagle dogs. The implants of five animals each were evaluated after a healing period of 1 month and 3 months, during which sequential fluorochrome labelling of bone formation was performed. Bone formation was evaluated by morphometric measurement of the newly formed bone around the implant and the percentage of implant bone contact. After 1 month there was only little bone/implant contact, varying between 2.6 and 6.7% in the cortical bone and 4.4 and 5.7% in the cancellous bone, with no significant differences between the three types of implants. After 3 months, implants with polished surfaces exhibited 26.5 and 31.2% contact in the cortical and cancellous bone, respectively, while collagen-coated implants had 19.5 and 28.4% bone contact in these areas. Implants with RGD coating showed the highest values with 42.1% and 49.7%, respectively. Differences between the surface types as such were not significant, but the increase in bone/implant contact from 1 to 3 months postoperatively was significant only in the group of RGD-coated implants (P = 0.008 and P = 0.000). The results of this pilot study thus provide only weak evidence that coating of titanium implants with RGD peptides in the present form and dosage may increase periimplant bone formation in the alveolar process. The results therefore require further verification in a modified experimental setting.
本研究的目的是分析钛种植体的有机涂层对种植体周围骨形成和骨/种植体接触的影响。使用了三种类型的种植体：（i）表面抛光的Ti6Al4V种植体（对照1）；（ii）胶原涂层的Ti6Al4V种植体（对照2）；（iii）胶原涂层且共价结合RGD肽的Ti6Al4V种植体。所有种植体的横截面均为方形，斜径为4.6 mm，通过压入配合的方式植入10只比格犬下颌骨中直径为4.6 mm的环钻钻孔内。在1个月和3个月的愈合期后，对每组5只动物的种植体进行评估，在此期间对骨形成进行连续的荧光染料标记。通过对种植体周围新形成骨的形态测量以及种植体骨接触百分比来评估骨形成情况。1个月后，骨/种植体接触很少，皮质骨中的接触率在2.6%至6.7%之间，松质骨中的接触率在4.4%至5.7%之间，三种类型的种植体之间无显著差异。3个月后，表面抛光的种植体在皮质骨和松质骨中的接触率分别为26.5%和31.2%，而胶原涂层种植体在这些区域的骨接触率为19.5%和28.4%。RGD涂层种植体的接触率最高，分别为42.1%和49.7%。表面类型之间的差异本身并不显著，但术后1至3个月骨/种植体接触的增加仅在RGD涂层种植体组中显著（P = 0.008和P = 0.000）。因此，这项初步研究的结果仅提供了微弱的证据，表明以目前的形式和剂量用RGD肽包被钛种植体可能会增加牙槽突种植体周围的骨形成。因此，结果需要在改进的实验环境中进一步验证。
