Leigh-Firbank Elizabeth C, Minihane Anne M, Leake David S, Wright John W, Murphy Margaret C, Griffin Bruce A, Williams Christine M
The Hugh Sinclair Unit of Human Nutrition, The University of Reading, UK.
Br J Nutr. 2002 May;87(5):435-45. doi: 10.1079/BJNBJN2002556.
Fish-oil supplementation can reduce circulating triacylglycerol (TG) levels and cardiovascular risk. This study aimed to assess independent associations between changes in platelet eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and fasting and postprandial (PP) lipoprotein concentrations and LDL oxidation status, following fish-oil intervention. Fifty-five mildly hypertriacylglycerolaemic (TG 1.5-4.0 mmol/l) men completed a double-blind placebo controlled cross over study, where individuals consumed 6 g fish oil (3 g EPA+DHA) or 6 g olive oil (placebo)/d for two 6-week intervention periods, with a 12-week wash-out period in between. Fish-oil intervention resulted in a significant increase in the platelet phospholipid EPA (+491 %, P<0.001) and DHA (+44 %, P<0.001) content and a significant decrease in the arachidonic acid (-10 %, P<0.001) and gamma-linolenic acid (-24 %, P<0.001) levels. A 30 % increase in ex vivo LDL oxidation (P<0.001) was observed. In addition, fish oil resulted in a significant decrease in fasting and PP TG levels (P<0.001), PP non-esterified fatty acid (NEFA) levels, and in the percentage LDL as LDL-3 (P=0.040), and an increase in LDL-cholesterol (P=0.027). In multivariate analysis, changes in platelet phospholipid DHA emerged as being independently associated with the rise in LDL-cholesterol, accounting for 16 % of the variability in this outcome measure (P=0.030). In contrast, increases in platelet EPA were independently associated with the reductions in fasting (P=0.046) and PP TG (P=0.023), and PP NEFA (P=0.015), explaining 15-20 % and 25 % of the variability in response respectively. Increases in platelet EPA+DHA were independently and positively associated with the increase in LDL oxidation (P=0.011). EPA and DHA may have differential effects on plasma lipids in mildly hypertriacylglycerolaemic men.
补充鱼油可降低循环中的三酰甘油（TG）水平及心血管疾病风险。本研究旨在评估鱼油干预后，血小板中二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的变化与空腹及餐后（PP）脂蛋白浓度以及低密度脂蛋白（LDL）氧化状态之间的独立关联。55名轻度高甘油三酯血症（TG 1.5 - 4.0 mmol/L）男性完成了一项双盲安慰剂对照交叉研究，研究中个体在两个为期6周的干预期内每日服用6克鱼油（3克EPA + DHA）或6克橄榄油（安慰剂），中间有12周的洗脱期。鱼油干预使血小板磷脂中EPA含量显著增加（+491%，P < 0.001），DHA含量显著增加（+44%，P < 0.001），花生四烯酸水平显著降低（-10%，P < 0.001），γ-亚麻酸水平显著降低（-24%，P < 0.001）。观察到离体LDL氧化增加30%（P < 0.001）。此外，鱼油使空腹及餐后TG水平显著降低（P < 0.001），餐后非酯化脂肪酸（NEFA）水平降低，LDL作为LDL - 3的百分比降低（P = 0.040），LDL胆固醇增加（P = 0.027）。在多变量分析中，血小板磷脂DHA的变化被证明与LDL胆固醇升高独立相关，占该结果指标变异性的16%（P = 0.030）。相反，血小板EPA的增加与空腹（P = 0.046）及餐后TG（P = 0.023）和餐后NEFA（P = 0.015）的降低独立相关，分别解释了反应变异性的15 - 20%和25%。血小板EPA + DHA的增加与LDL氧化增加独立且呈正相关（P = 0.011）。EPA和DHA对轻度高甘油三酯血症男性的血脂可能有不同影响。
