Barbour Suzanne E, Ishihara Yuichi, Fakher Mohammed, Al-Darmaki Salma, Caven Timothy H, Shelburne C P, Best Al M, Schenkein Harvey A, Tew John G
Department of Microbiology and Immunology, Virginia Commonwealth University School of Dentistry, Richmond, Virginia 23298-0678, USA.
Infect Immun. 2002 Jun;70(6):2780-6. doi: 10.1128/IAI.70.6.2780-2786.2002.
Several lines of evidence indicate that the monocytes of subjects with localized juvenile periodontitis (LJP) are functionally distinct from cells of age- and race-matched nonperiodontitis (NP) subjects. Among the abnormalities are the propensity to secrete large amounts of prostaglandin E(2) and the induction of immunoglobulin G2 (IgG2) antibodies. The experiments described here were performed to further characterize the LJP monocytes and to determine if these cells mature differently than NP monocytes. When adherent monocytes from LJP subjects were cultured in the presence of human serum, both macrophages and cells with the morphology of immature monocyte-derived dendritic cells (MDDC) were observed. Within 4 days the prevalence of the immature MDDC was approximately twofold higher in LJP cultures than in NP cultures. In addition to their dendritic morphology, these cells were CD11c(+) and CD14(-) or CD14(low) and stimulated potent autologous mixed leukocyte reactions, consistent with differentiation to the MDDC phenotype. Like LJP monocytes, cultures of MDDC generated with interleukin-4 and granulocyte-macrophage colony-stimulating factor selectively induced IgG2 in cultures of pokeweed mitogen-stimulated NP leukocytes. Together, these data suggest that the monocytes of LJP subjects have a propensity to differentiate into MDDC and that this differentiation may be related to the high levels of IgG2 that are observed in the sera of LJP subjects. As high levels of circulating IgG2 are correlated with less severe disease, the propensity of LJP monocytes to differentiate into MDDC may have important implications for both the host response against oral pathogens and the progression of LJP.
多项证据表明，局限性青少年牙周炎（LJP）患者的单核细胞在功能上与年龄和种族匹配的非牙周炎（NP）患者的细胞不同。这些异常包括分泌大量前列腺素E2的倾向以及免疫球蛋白G2（IgG2）抗体的诱导。此处描述的实验旨在进一步表征LJP单核细胞，并确定这些细胞的成熟过程是否与NP单核细胞不同。当在人血清存在的情况下培养LJP患者的贴壁单核细胞时，观察到了巨噬细胞和具有未成熟单核细胞衍生树突状细胞（MDDC）形态的细胞。在4天内，LJP培养物中未成熟MDDC的患病率比NP培养物中高约两倍。除了其树突状形态外，这些细胞为CD11c(+)和CD14(-)或CD14(低)，并刺激强烈的自体混合白细胞反应，这与向MDDC表型的分化一致。与LJP单核细胞一样，用白细胞介素-4和粒细胞-巨噬细胞集落刺激因子产生的MDDC培养物在商陆有丝分裂原刺激的NP白细胞培养物中选择性诱导IgG2。总之，这些数据表明LJP患者的单核细胞有分化为MDDC的倾向，并且这种分化可能与LJP患者血清中观察到的高水平IgG2有关。由于循环中高水平的IgG2与病情较轻相关，LJP单核细胞分化为MDDC的倾向可能对宿主针对口腔病原体的反应和LJP的进展都有重要影响。
