Giovannini E, Rosi G
Br J Haematol. 1975 Jan;29(1):129-35. doi: 10.1111/j.1365-2141.1975.tb01806.x.
The subcellular localization of erythrostimulant factors contained in the kidney and spleen of the rabbit have been studied. This was done by subjecting homogenates to fractionation by centrifugation and assessing the erythrostimulant capacity of the hypotonic extracts prepared from the various fractions thus obtained. The results of these studies show that the erythrostimulant factors are present in the light mitochondrial and microsomal fractions of the normal kidney. In conditions of anaemia they are to be found not only in the above-mentioned fractions but also in the mitochondrial fraction. By contrast, in the spleen such factors are present only in the mitochondrial fraction. It is likely that the different localization of the erythrostimulant factors in the kidney and in the spleen of the rabbit may be closely connected with the different functions performed by these organs in the humoral regulation of erythropoiesis.
对家兔肾脏和脾脏中所含促红细胞生成因子的亚细胞定位进行了研究。通过对匀浆进行离心分级分离，并评估从由此获得的各个级分制备的低渗提取物的促红细胞生成能力来完成此项研究。这些研究结果表明，促红细胞生成因子存在于正常肾脏的轻线粒体和微粒体级分中。在贫血状态下，不仅在上述级分中能发现它们，在线粒体级分中也能发现。相比之下，在脾脏中，这些因子仅存在于线粒体级分中。家兔肾脏和脾脏中促红细胞生成因子的不同定位可能与这些器官在红细胞生成的体液调节中所执行的不同功能密切相关。
