England J M, Down M C
Br J Haematol. 1975 Jul;30(3):365-70. doi: 10.1111/j.1365-2141.1975.tb00552.x.
When trace quantities of labelled albumin have been added to blood, the packed cell volume (PCV) may be determined by isotope counting using the relationship: (plasma counts-blood counts)/(plasma counts). This method for measuring the PCV is not affected by plasma trapping between the red cells and the results do not depend upon the centrifugation conditions. The PCV determination with labelled albumin has a high precision (coefficient of variation = 0.9%) and there is very good agreement with the centrifugation PCV using Wintrobe tubes after this measurement has been corrected for plasma trapped between the red cells. No significant compression of the red cells occurred when the blood samples were centrifugated in Wintrobe PCV tubes.
当向血液中添加微量标记白蛋白后,可利用以下关系通过同位素计数来测定血细胞压积(PCV):(血浆计数-血液计数)/(血浆计数)。这种测量PCV的方法不受红细胞间血浆滞留的影响,其结果也不取决于离心条件。用标记白蛋白测定PCV具有很高的精度(变异系数=0.9%),并且在对红细胞间滞留的血浆进行校正后,与使用温氏管离心法测定的PCV非常吻合。当血液样本在温氏PCV管中离心时,红细胞未出现明显压缩。