四氯化碳诱导的大鼠肝硬化中巨噬细胞、肌成纤维细胞和肥大细胞的动力学
Kinetics of macrophages, myofibroblasts and mast cells in carbon tetrachloride-induced rat liver cirrhosis.
作者信息
Jeong Won-Il, Lee Cha-Soo, Park Sang-Joon, Chung Jae-Yong, Jeong Kyu-Shik
机构信息
Department of Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu City, South Korea.
出版信息
Anticancer Res. 2002 Mar-Apr;22(2A):869-77.
BACKGROUND
Recently, activation of macrophages, myofibroblasts and mast cells have been thought to be associated with liver fibrosis. The present study investigated the kinetics of these cells and the numeric relationship in carbon tetrachloride (CCl4)-induced rat liver cirrhosis.
MATERIALS AND METHODS
Macrophages and myofibroblasts were observed with immunohistochemistry and mast cells were detected by toluidine blue.
RESULTS
Mast cells increased in proportion to the development of fibrosis with a maximum number on week 14. The numbers of macrophages and myofibroblasts increased during weeks 0 to 10 but decreased from weeks 12 to 14.
CONCLUSION
The numbers of macrophages and myofibroblasts increased in the development of fibrosis but decreased when cirrhosis was induced. However, mast cells increased in both. Therefore, there was a numerical relationship among the numbers of macrophages, myofibroblasts and mast cells in liver fibrosis but none between the former two cells and the mast cells in liver cirrhosis.
背景
最近，巨噬细胞、肌成纤维细胞和肥大细胞的激活被认为与肝纤维化有关。本研究调查了四氯化碳（CCl4）诱导的大鼠肝硬化中这些细胞的动力学变化以及数量关系。
材料与方法
采用免疫组织化学法观察巨噬细胞和肌成纤维细胞，用甲苯胺蓝检测肥大细胞。
结果
肥大细胞数量随纤维化发展而增加，在第14周达到最大值。巨噬细胞和肌成纤维细胞数量在0至10周增加，但在12至14周减少。
结论
巨噬细胞和肌成纤维细胞数量在纤维化发展过程中增加，但在诱导肝硬化时减少。然而，肥大细胞在两者中均增加。因此，肝纤维化中巨噬细胞、肌成纤维细胞和肥大细胞数量之间存在数量关系，但前两者细胞与肝硬化中的肥大细胞之间不存在数量关系。
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