Jeong Won-Il, Do Sun-Hee, Jeong Da-Hee, Hong Il-Hwa, Park Jin-Kyu, Ran Ki-Mi, Yang Hai-Jie, Yuan Dong-Wei, Kim Soon-Bok, Cha Myung-Sook, Jeong Kyu-Shik
Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Anticancer Res. 2006 Sep-Oct;26(5A):3517-26.
The involvement of whether macrophages and mast cells in the kinetics of matrix metalloproteinases-1 (MMP-1), MMP-3 and MMP-9, tissue inhibitors of metalloproteinases-1 (TIMP-1) and TIMP-2 in hepatic fibrosis/cirrhosis was examined.
The MMPs and TIMPs were examined by histopathology, immunohistochemistry and immunoblotting using carbon tetrachloride-induced fibrotic rat liver.
MMP-1 increased in proportion to the development of fibrosis and reached maximum at week 14. In the first four weeks, MMP-3 expression was mainly observed in many hepatocytes. At week 8, the macrophages in the fibrous septa, as well as hepatocytes, expressed MMP-3. TIMP-1 and -2 progressively increased throughout the experimental periods. The MMP-1 expression in the mast cells, however, did not decrease the degree of liver cirrhosis.
MMP-1, TIMP-1 and -2 expressions increased in the late stages of fibrosis and cirrhosis. During recovery, the MMP-3 expression of macrophages greatly increased in the unresolved fibrous septa.
研究了巨噬细胞和肥大细胞是否参与基质金属蛋白酶-1（MMP-1）、MMP-3和MMP-9、金属蛋白酶组织抑制剂-1（TIMP-1）和TIMP-2在肝纤维化/肝硬化过程中的动力学变化。
使用四氯化碳诱导的纤维化大鼠肝脏，通过组织病理学、免疫组织化学和免疫印迹法检测MMPs和TIMPs。
MMP-1的增加与纤维化的发展成比例，并在第14周达到最大值。在最初的四周内，MMP-3的表达主要在许多肝细胞中观察到。在第8周，纤维间隔中的巨噬细胞以及肝细胞表达MMP-3。在整个实验期间，TIMP-1和-2逐渐增加。然而，肥大细胞中的MMP-1表达并未降低肝硬化的程度。
在纤维化和肝硬化的后期，MMP-1、TIMP-1和-2的表达增加。在恢复过程中，未解决的纤维间隔中巨噬细胞的MMP-3表达大大增加。
