Piza A T, Pieri K M S, Lusa G M, Caporale G M M, Terreran M T, Machado L A, Zanetti C R
Vallée S.A., São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz. 2002 Mar;97(2):265-8. doi: 10.1590/s0074-02762002000200022.
One of the methods used for controlling cattle rabies in Brazil consists of vaccination. Sometimes, however, rabies occurs in cattle supposedly protected. Since rabies vaccine batches are officially controlled by tests performed on laboratory animals, it is questionable whether the minimal mandatory requirements really correspond to immunogenicity in the target species. We have analyzed the association among potencies of rabies vaccines tested by the NIH test, the contents and form (free-soluble or virus-attached) of rabies glycoprotein (G) in the vaccine batches, and the virus-neutralizing antibodies (VNA) titers elicited in cattle. No correlation was found between G contents in the vaccine batches and the NIH values, whatever the presentation of G. There was no correlation either between NIH values and VNA titers elicited in cattle. There was, however, a positive correlation (r = 0.8681; p = 0.0001) between the amounts of virion-attached G present in the vaccine batches and VNA elicited in cattle. This was not observed when the same analysis was performed with total-glycoprotein or free-soluble glycoprotein. The study demonstrated that NIH values can not predict the effect of the immunogen in cattle. On the other hand, the quantification of virus-attached rabies glycoprotein has a strong correlation with VNA elicited in cattle.
巴西控制牛狂犬病的方法之一是接种疫苗。然而，有时在本应受到保护的牛群中也会出现狂犬病。由于狂犬病疫苗批次是通过对实验动物进行的测试来进行官方管控的，所以最低强制要求是否真的与目标物种的免疫原性相符存在疑问。我们分析了通过美国国立卫生研究院（NIH）测试检测的狂犬病疫苗效力、疫苗批次中狂犬病糖蛋白（G）的含量和形式（游离可溶或病毒附着）以及牛体内产生的病毒中和抗体（VNA）滴度之间的关联。无论G的呈现形式如何，疫苗批次中的G含量与NIH值之间均未发现相关性。NIH值与牛体内产生的VNA滴度之间也没有相关性。然而，疫苗批次中存在的病毒附着G的量与牛体内产生的VNA之间存在正相关（r = 0.8681；p = 0.0001）。当对总糖蛋白或游离可溶糖蛋白进行相同分析时，未观察到这种情况。该研究表明，NIH值无法预测免疫原对牛的效果。另一方面，病毒附着狂犬病糖蛋白的定量与牛体内产生的VNA有很强的相关性。
