Liao L, Lei M, Han X, Chen H, Fan C
Department of Endocrinology, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Hunan Yi Ke Da Xue Xue Bao. 1999;24(3):263-6.
To clarify the relationship between serum angiotensin I-converting enzyme (ACE) activity and Type II diabetic patients with or without diabetic nephropathy. We examined 36 healthy controls, 58 Type II diabetic patients without diabetic nephropathy, and 50 Type II diabetic with diabetic nephropathy. The diabetic duration of all of Type II diabetic patients was more than 5 years. All patients matched well in age and BMI. An insertion/deletion polymorphism of ACE gene was identified by polymerase chain reaction(PCR). Serum ACE activity was determined using spectrophotometry. Although the distribution of DD, ID, and II genotypes of the ACE gene did not differ among the three groups, serum ACE activity was significantly higher in Type II diabetic patients with diabetic nephropathy than in that without diabetic nephropathy(P < 0.05), especially in the groups with D allele of ACE gene. Serum ACE activity had no difference between healthy controls and Type II diabetic patients without diabetic nephropathy. There is no association between serum ACE activity and Type II diabetic patients without diabetic nephropathy in any genotype of ACE gene. The increasing serum ACE activity plays a role in the initiation of diabetic nephropathy or may serve as a risk marker for later development of overt diabetic nephropathy in Type II diabetic patients.
为阐明血清血管紧张素I转换酶（ACE）活性与伴有或不伴有糖尿病肾病的II型糖尿病患者之间的关系。我们检测了36名健康对照者、58名无糖尿病肾病的II型糖尿病患者以及50名患有糖尿病肾病的II型糖尿病患者。所有II型糖尿病患者的糖尿病病程均超过5年。所有患者在年龄和体重指数方面匹配良好。通过聚合酶链反应（PCR）鉴定ACE基因的插入/缺失多态性。使用分光光度法测定血清ACE活性。尽管ACE基因的DD、ID和II基因型在三组中的分布没有差异，但患有糖尿病肾病的II型糖尿病患者的血清ACE活性显著高于无糖尿病肾病的患者（P<0.05），尤其是在具有ACE基因D等位基因的组中。健康对照者与无糖尿病肾病的II型糖尿病患者之间的血清ACE活性没有差异。在ACE基因的任何基因型中，血清ACE活性与无糖尿病肾病的II型糖尿病患者之间均无关联。血清ACE活性升高在II型糖尿病患者糖尿病肾病的发生中起作用，或可能作为显性糖尿病肾病后期发展的风险标志物。
