Degirmenci I, Kebapci N, Basaran A, Efe B, Gunes H V, Akalin A, Kurt H, Urhan M, Demirustu C
Department of Medical Biology, Medical Faculty, Osmangazi University, Eskisehir, Turkey.
Int J Clin Pract. 2005 Oct;59(10):1137-42. doi: 10.1111/j.1368-5031.2005.00586.x.
We aimed to investigate the angiotensin-converting enzyme (ACE) gene polymorphism, ACE activity and their associations with diabetic complications in Turkish patients with type 2 diabetes mellitus. A total of 143 patients and 133 controls were screened for ACE gene I/D polymorphism by using polymerase chain reaction. Serum ACE activities were determined spectrophotometrically. There was no significant difference in the distribution of ACE I/D genotypes between patients and controls. The patients with DD genotype had a higher ACE activity than those with ID and II. Hypertensive diabetic patients with DD genotype had higher ACE activities than those with ID and II. There was no significant difference in the distribution of ACE I/D genotypes between patients with and without nephropathy, retinopathy and hypertension except for patients with and without neuropathy. In patients with DD genotype, creatinine clearance correlated with duration of diabetes. The grade of retinopathy was correlated with duration of diabetes in DD and ID genotypes. The highest ACE activity was measured in hypertensive diabetics with DD genotype. ID genotype was suggested to be a risk factor and II was suggested to be protective for diabetic neuropathy. The DD and ID genotypes might be a predictor for the development of retinopathy in relation to duration of diabetes.
我们旨在研究土耳其2型糖尿病患者血管紧张素转换酶（ACE）基因多态性、ACE活性及其与糖尿病并发症的关联。通过聚合酶链反应对总共143例患者和133例对照进行ACE基因I/D多态性筛查。采用分光光度法测定血清ACE活性。患者和对照之间ACE I/D基因型分布无显著差异。DD基因型患者的ACE活性高于ID和II基因型患者。DD基因型的高血压糖尿病患者的ACE活性高于ID和II基因型患者。除有或无神经病变的患者外，有或无肾病、视网膜病变和高血压的患者之间ACE I/D基因型分布无显著差异。在DD基因型患者中，肌酐清除率与糖尿病病程相关。在DD和ID基因型中，视网膜病变程度与糖尿病病程相关。在DD基因型的高血压糖尿病患者中测得最高的ACE活性。ID基因型被认为是糖尿病神经病变的危险因素，而II基因型被认为具有保护作用。DD和ID基因型可能是与糖尿病病程相关的视网膜病变发生的预测指标。
