Qiao Lei, Chen Xiao-Hui, Bi Kai-Shun
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(18):1900-2.
To develop a RP-HPLC method for the determination of the content of macrozamin in Rhizoma Heterosmilacis Japonicae.
A Century C18 AQ column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase consisted of water (4:96). The flow rate was 1.0 mL x min(-1). The detection wavelength was set at 215 nm, and the column temperature was 35 degrees C.
The calibration curve was linear (r = 0.999 8) in the range of 19.12 - 382.4 microg x mL(-1) for macrozamin, the average recovery of the method was 99.5%, with RSD 2.1% (n = 9).
This method can be used for the quality study of Rhizoma Heterosmilacis Japonicae.
建立测定土茯苓中落新妇苷含量的反相高效液相色谱法。
采用世纪C18 AQ柱（4.6 mm×250 mm，5μm），流动相为水（4∶96）。流速为1.0 mL·min-1。检测波长设定为215 nm，柱温为35℃。
落新妇苷在19.12～382.4μg·mL-1范围内线性关系良好（r = 0.999 8），该方法的平均回收率为99.5%，相对标准偏差为2.1%（n = 9）。
该方法可用于土茯苓的质量研究。
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