[反相高效液相色谱法测定酸枣仁中酸枣仁皂苷A的含量]
[Determination of jujubaside A in semen ziziphi spinosae by RP-HPLC].
作者信息
Li Y J, Che Z T, Bi K S, Xu Z M, Li M
机构信息
Shenyang Pharmaceutical University, Shenyang 110015, Liaoning, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2001 May;26(5):309-10.
PMID:12528518
Abstract
OBJECTIVE
To develop a new method for the determination of Jujubaside A in Semen Ziziphi Spinosae.
METHOD
A RP-HPLC method was set up, using Hypersil-18 column, acetonitrile: water(30:70) as mobile phase with a flow rate of 0.8 ml.min-1.
RESULT
The calibration curve was linear in the range of 0.076-0.456 mg. The average recovery was 93.5%.
CONCLUSION
The method is appropriate for the determination of Jujubaside A.
摘要
目的
建立一种测定酸枣仁中酸枣仁皂苷A的新方法。
方法
采用RP-HPLC法，以Hypersil-18柱，乙腈∶水（30∶70）为流动相，流速为0.8 ml·min-1。
结果
校准曲线在0.076～0.456 mg范围内呈线性。平均回收率为93.5%。
结论
该方法适用于酸枣仁皂苷A的测定。
Zotero 插件