Hu Y F, Mao X H, Lu C D
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031.
Shi Yan Sheng Wu Xue Bao. 1998 Sep;31(3):265-71.
By homo-recombination with yeast intergrating plasmids, a serial of haploid mutants of budding yeast YPH499 had been constructed, which included pho85 delta strain YPH600, pho85 delta cln1 delta strain YPH610, cln1 delta cln2 delta strain YPH640 and galactose inducible strain YPH630 (pho85 delta cln1 delta cln2 delta (GAL1-10PHO85)). By analyzing the growth rate of different strains, we concluded that PHO85 gene have greater influence than CLN1 and CLN2 genes on cell growth control. After transferred from galactose media to glucose media, the tri-mutant cells collected at intervals were observed with microscope and analyzed by FACS. The results showed that the tri-mutant cells arrest in G1 phase when they were transferred to glucose media.
通过与酵母整合质粒的同源重组，构建了一系列芽殖酵母YPH499的单倍体突变体，其中包括pho85Δ菌株YPH600、pho85Δcln1Δ菌株YPH610、cln1Δcln2Δ菌株YPH640和半乳糖诱导菌株YPH630（pho85Δcln1Δcln2Δ（GAL1-10PHO85））。通过分析不同菌株的生长速率，我们得出结论，PHO85基因对细胞生长控制的影响比CLN1和CLN2基因更大。从半乳糖培养基转移到葡萄糖培养基后，每隔一段时间收集的三突变体细胞用显微镜观察并用FACS分析。结果表明，三突变体细胞转移到葡萄糖培养基时停滞在G1期。
