Sambuk E V, Popova Iu G, Padkina M V
Biological Research Institute, St. Petersburg State University, St. Petersburg, 198904 Russia.
Genetika. 2003 Jan;39(1):18-24.
Chaperones are known to play an important role in complexation of cyclin-dependent kinases with cyclins. In yeast cells growing in the presence of phosphate, cyclin-dependent kinase Pho85p and cyclin Pho80p form a complex and phosphorylate activator Pho4p. As a result, Pho4p is exported from the nucleus, and the PHO5 gene is not transcribed. The mutations suppressing the pho85 mutation were analyzed in order to identify genes which code for chaperones involved in the formation of the Pho80p-Pho85p complex in the presence of environmental phosphate. Dominant mutations DSP1, DSP2, and DSP4-6 were found. It is shown that the DSP1 gene is 2.1 cM away from the PHO85 gene on chromosome XVI and probably coincides with the EGD1 gene coding for a chaperone.
已知伴侣蛋白在细胞周期蛋白依赖性激酶与细胞周期蛋白的复合过程中发挥重要作用。在磷酸盐存在下生长的酵母细胞中，细胞周期蛋白依赖性激酶Pho85p和细胞周期蛋白Pho80p形成复合物并磷酸化激活因子Pho4p。结果，Pho4p从细胞核中输出，PHO5基因不被转录。为了鉴定在环境磷酸盐存在下参与Pho80p-Pho85p复合物形成的伴侣蛋白编码基因，对抑制pho85突变的突变进行了分析。发现了显性突变DSP1、DSP2和DSP4-6。结果表明，DSP1基因在第十六号染色体上距离PHO85基因2.1 cM，可能与编码伴侣蛋白的EGD1基因一致。
