Friedrich Michael, Meyberg Roland, Axt-Fliedner Roland, Villena-Heinsen Carlos, Tilgen Wolfgang, Schmidt Werner, Reichrath Jörg
Department of Gynecology, University Hospital of Saarland, Homburg/Saar, Germany.
Anticancer Res. 2002 Jan-Feb;22(1A):299-304.
The aim of this study was to analyze whether VDR status is a prognostic factor that may be of importance for the assessment of recurrence in cervical cancer.
VDR-, Ki-67- and p53-status were analyzed immunohistochemically in cervical cancer patients (n=50) and in benign cervical tissue (n=15). The histopathological data of the tumours were evaluated. RNA was extracted from normal cervical tissue (n=4) and cervical carcinomas (n=8) using the method of Chomczynski. RNA was reverse-transcribed and RNA-levels were semiquantitatively detected by PCR.
The expression of VDR was significantly increased in cervical cancer compared to normal cervical tissue on the protein-level but not on the RNA-level. No statistically significant correlations were found comparing VDR status with histopathological data (tumour stage, lymph node status, grading, histological tumour type), with the expression of the proliferation marker Ki-67 and of the tumour suppressor gene p53.
Our findings indicate that VDR protein expression is not a prognostic factor in cervical cancer. The strong I/DR immunoreactivity that we observed in cervical cancer specimens supports the body of evidence that cervical cancer may be a target for therapeutically applied vitamin D analogues.
本研究旨在分析维生素D受体（VDR）状态是否为宫颈癌复发评估中可能具有重要意义的预后因素。
采用免疫组织化学方法分析50例宫颈癌患者及15例宫颈良性组织中VDR、Ki-67和p53的状态。对肿瘤的组织病理学数据进行评估。采用Chomczynski法从4例正常宫颈组织和8例宫颈癌组织中提取RNA。RNA进行逆转录，并通过聚合酶链反应（PCR）半定量检测RNA水平。
与正常宫颈组织相比，宫颈癌组织中VDR在蛋白水平上表达显著增加，但在RNA水平上无明显变化。VDR状态与组织病理学数据（肿瘤分期、淋巴结状态、分级、组织学肿瘤类型）、增殖标志物Ki-67及肿瘤抑制基因p53的表达之间未发现统计学显著相关性。
我们的研究结果表明，VDR蛋白表达不是宫颈癌的预后因素。我们在宫颈癌标本中观察到的强VDR免疫反应性支持了宫颈癌可能是治疗性应用维生素D类似物的靶点这一证据。
