Silva Júnior Aurelício N, Pinheiro Antonio L B, Oliveira Marília G, Weismann Ruben, Ramalho Luciana Maria Pedreira, Nicolau Renata Amadei
School of Dentistry, Postgraduate Programe on Oral and Maxillofacial Surgery, Pontifícia Universidade Católica do Rico Grande do Sul, Porto Alegre, Brazil.
J Clin Laser Med Surg. 2002 Apr;20(2):83-7. doi: 10.1089/104454702753768061.
The aim of this study was to evaluate morphometrically the amount of newly formed bone after GaAlAs laser irradiation of surgical wounds created in the femur of rats.
Low-level laser therapy (LLLT) has been used in several medical specialties because of its biomodulatory effects on different biological tissues. However, LLLT is still controversial because of contradictory reports. This is a direct result of the different methodologies used in these works.
In this study, 40 Wistar rats were divided into four groups of 10 animals each: group A (12 sessions, 4.8 J/cm2 per session, observation time of 28 days); group C (three sessions, 4.8 J/cm2 per session, observation time of 7 days). Groups B and D acted as nonirradiated controls. The specimens were routinely processed to wax and cut at 6-microm thickness and stained with H&E. For computerized morphometry, Imagelab software was used.
Computerized morphometry showed a significant difference between the areas of mineralized bone in groups C and D (p = 0.017). There was no difference between groups A and B (28 days; p = 0.383).
It is concluded that, under this experimental condition, LLLT increased bone repair at early bone healing.
本研究旨在通过形态计量学评估在大鼠股骨上制造手术伤口后，经GaAlAs激光照射后新形成骨的量。
低强度激光疗法（LLLT）因其对不同生物组织的生物调节作用已被应用于多个医学专业。然而，由于报道相互矛盾，LLLT仍存在争议。这是这些研究中使用的不同方法直接导致的结果。
在本研究中，40只Wistar大鼠被分为四组，每组10只动物：A组（12次照射，每次4.8 J/cm²，观察时间28天）；C组（3次照射，每次4.8 J/cm²，观察时间7天）。B组和D组作为未照射对照组。标本常规处理成蜡块，切成6微米厚，并用苏木精和伊红染色。使用Imagelab软件进行计算机形态计量学分析。
计算机形态计量学显示C组和D组矿化骨面积有显著差异（p = 0.017）。A组和B组之间（28天；p = 0.383）无差异。
得出结论，在本实验条件下，LLLT在早期骨愈合时促进了骨修复。
