German A C, Shankland G S, Edwards J, Flach E J
Veterinary Science Unit, Institute of Zoology, Dunstable, Bedfordshire.
Vet Rec. 2002 Apr 20;150(16):513-8. doi: 10.1136/vr.150.16.513.
Aspergillosis is a significant cause of mortality in captive penguins (Sphenisciformes). An indirect ELISA for the detection of Aspergillus fumigatus-specific immunoglobulin in penguins was developed and standardised by making use of a family-specific antiserum (anti-Aptenodyptes patagonica patagonicus). The results were calculated quantitatively as ELISA units, derived by polynomial regression analysis, and semi-quantitatively as end titres. Serum samples from 61 captive penguins were tested with the assay, and the results were compared with those obtained by counterimmunoelectrophoresis (CIE). The ELISA results correlated with the CIE results only when end titres were reported (R(s) = -0.676, P < 0.002). Fifty-seven of the penguins (93 per cent) were seropositive, but the detection of immunoglobulin did not correlate with clinical disease. At Whipsnade Wild Animal Park, Humboldt's penguins (Spheniscus humboldti) demonstrated higher seropositivity than king penguins (Aptenodyptes patagonicapatagonicus) (P = 0.022), but Humboldt's penguins at Fota Wildlife Park had a significantly higher seropositivity than Humboldt's penguins at Whipsnade (P = 0.035).
曲霉病是圈养企鹅（企鹅目）死亡的一个重要原因。利用一种种属特异性抗血清（抗巴塔哥尼亚企鹅）开发并标准化了一种用于检测企鹅中烟曲霉特异性免疫球蛋白的间接酶联免疫吸附测定（ELISA）。结果通过多项式回归分析以ELISA单位进行定量计算，并以终点滴度进行半定量计算。用该检测方法对61只圈养企鹅的血清样本进行了检测，并将结果与对流免疫电泳（CIE）所得结果进行了比较。仅在报告终点滴度时，ELISA结果与CIE结果相关（R(s)= -0.676，P < 0.002）。57只企鹅（93%）血清呈阳性，但免疫球蛋白的检测与临床疾病无关。在惠普斯奈德野生动物园，洪堡企鹅（洪氏环企鹅）的血清阳性率高于帝企鹅（巴塔哥尼亚企鹅）（P = 0.022），但福塔野生动物园的洪堡企鹅血清阳性率显著高于惠普斯奈德的洪堡企鹅（P = 0.035）。
