Jones Francis, Lu Zonghuan, Elmore Bill B
Chemical and Environmental Engineering, The University of Tennessee at Chattanooga, 37403-2598, USA.
Appl Biochem Biotechnol. 2002 Spring;98-100:627-40. doi: 10.1385/abab:98-100:1-9:627.
This study involves a novel method for immobilized enzyme catalysis. The focus of the work was to design and construct a microscale bioreactor using microfabrication techniques traditionally employed within the semiconductor industry. Enzymes have been immobilized on the microreactor walls by incorporating them directly into the wall material. Fabricated microchannels have cross-sectional dimensions on the order of hundreds of micrometers, constructed using polydimethylsiloxane cast on silicon/SU-8 molds. The resulting ratio of high surface area to volume creates an efficient, continuous-flow reaction system. Transverse features also containing enzymes were molded directly into the channels.
本研究涉及一种用于固定化酶催化的新方法。工作重点是利用半导体行业传统采用的微加工技术设计和构建一个微型生物反应器。通过将酶直接掺入壁材料中，已将其固定在微反应器壁上。制造的微通道的横截面尺寸在数百微米量级，是使用浇铸在硅/SU-8模具上的聚二甲基硅氧烷构建的。由此产生的高表面积与体积之比创造了一个高效的连续流反应系统。还将含有酶的横向特征直接模制到通道中。
