Gillette Karin, Misra Vikram, Bratanich Ana
Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo 58105, USA.
Virus Genes. 2002 Mar;24(2):149-52. doi: 10.1023/a:1014520616362.
Bovine herpesvirus (BHV), a member of the subfamily Alphaherpesvirinae, is classified into neurovirulent and non-neurovirulent subtypes on a basis of differential neuropathogenicities. Transcription of viral immediate early (IE) genes during alphaherpesvirus gene expression, is mediated by a multi-component immediate early complex (IEC) integrated by the viral tegument protein alpha trans-inducing factor (alpha-tif), a host cell protein (HCF), and a host Octamer protein (Oct). In this paper, we present a sequence analysis of the alpha-tif of the encephalitic BHV subtype, bovine herpesvirus type 5 (BHV-5). Bovine herpesvirus type 1 (BHV-1) and BHV-5 alpha-tifs share 98% amino acid sequence homology. However, BHV-5 alpha-tif is 23 residues shorter at the amino terminus than BHV-1 alpha-tif. Amino acid alignment of the alpha-tifs of BHV-1 and BHV-5 with other alphaherpesviruses indicates areas of conserved motifs but also important differences located mainly at the amino and carboxyl termini.
牛疱疹病毒（BHV）是α疱疹病毒亚科的成员，根据不同的神经致病性分为神经毒力型和非神经毒力型亚型。在α疱疹病毒基因表达过程中，病毒立即早期（IE）基因的转录由一个多组分立即早期复合体（IEC）介导，该复合体由病毒被膜蛋白α反式诱导因子（α-tif）、一种宿主细胞蛋白（HCF）和一种宿主八聚体蛋白（Oct）整合而成。在本文中，我们展示了脑炎性BHV亚型——牛疱疹病毒5型（BHV-5）的α-tif的序列分析。牛疱疹病毒1型（BHV-1）和BHV-5的α-tif具有98%的氨基酸序列同源性。然而，BHV-5的α-tif在氨基末端比BHV-1的α-tif短23个残基。BHV-1和BHV-5的α-tif与其他α疱疹病毒的氨基酸比对表明，存在保守基序区域，但也存在主要位于氨基和羧基末端的重要差异。
