Engelhardt T, Keil G M
Institute of Molecular and Cellular Virology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany.
Virology. 1996 Nov 1;225(1):126-35. doi: 10.1006/viro.1996.0580.
The BHV-5 strain N569 (BHV-5/N569) homolog to the BHV-1 US4 gene was sequenced and characterized. RNA analyses showed that a 1.8-kb mRNA which contains the BHV-5/N569 US4 open reading frame initiates 55 nucleotides upstream from the predicted translational start codon and terminates 17 nucleotides downstream from the consensus sequence for polyadenylation. Comparison of the deduced amino acid sequences of the predicted US4 encoded proteins of BHV-5/N569 and BHV-1 strain Schönböken (BHV-1/Schö) revealed 75% identity. An antiserum, raised in rabbits after infection with a BHV-5/N569 US4 ORF expressing recombinant vaccinia virus, specifically precipitated a 65-kDa protein and a diffusely migrating protein species with an apparent molecular mass between 90 and > 240 kDa from the supernatant of BHV-5/ N569 infected cells. Treatment of immmunprecipitated proteins with chondroitinase AC demonstrated that the latter contains glycosaminoglycans. The mobility of the BHV-5/N569 US4 gene products was identical to the BHV-1 US4 ORF encoded glycoprotein G (gG) and glycoproteoglycan G (gpgG; G. M. Keil, T. Engelhardt, A. Karger, and M. Enz. J. Virol. 70, 3032-3038, 1996) and were therefore named BHV-5 gG and BHV-5 gpgG. Immunoprecipitations with sera from BHV-1 infected cattle indicated a type-specific immune response to gG, since these sera failed to react with vaccinia virus-expressed gG-5 but recognized vaccinia virus-expressed gG-1.
对与牛疱疹病毒1型（BHV-1）US4基因同源的牛疱疹病毒5型（BHV-5）毒株N569（BHV-5/N569）进行了测序和特性分析。RNA分析表明，一个包含BHV-5/N569 US4开放阅读框的1.8 kb mRNA在预测的翻译起始密码子上游55个核苷酸处起始，并在聚腺苷酸化共有序列下游17个核苷酸处终止。对BHV-5/N569和BHV-1毒株舍恩博肯株（BHV-1/Schö）预测的US4编码蛋白推导氨基酸序列的比较显示，二者具有75%的同一性。用表达BHV-5/N569 US4开放阅读框的重组痘苗病毒感染兔子后产生的抗血清，能从BHV-5/N569感染细胞的上清液中特异性沉淀出一种65 kDa的蛋白以及一种表观分子量在90至>240 kDa之间的弥散迁移蛋白条带。用软骨素酶AC处理免疫沉淀的蛋白表明，后者含有糖胺聚糖。BHV-5/N569 US4基因产物的迁移率与BHV-1 US4开放阅读框编码的糖蛋白G（gG）和糖蛋白聚糖G（gpgG；G.M.凯尔、T.恩格尔哈特、A.卡格尔和M.恩茨，《病毒学杂志》70:3032 - 3038，1996年）相同，因此分别命名为BHV-5 gG和BHV-5 gpgG。用BHV-1感染牛的血清进行免疫沉淀表明，对gG存在型特异性免疫反应，因为这些血清不能与痘苗病毒表达的gG-5反应，但能识别痘苗病毒表达的gG-1。
