Heine J W, Honess R W, Cassai E, Roizman B
J Virol. 1974 Sep;14(3):640-51. doi: 10.1128/JVI.14.3.640-651.1974.
The polypeptides from purified virions of a herpes simplex 1 (human herpes-virus 1) strain, F1, which had been passaged a limited number of times in cell culture after isolation, formed 33 bands on electrophoretic separation in polyacrylamide gels cross-linked with N, N'-diallyltartardiamide in contrast to a maximum resolution of only 24 to 25 bands in gels cross-linked with N, N'-methylenebisacrylamide. This increase in the number of bands was due chiefly to an improved separation of glycosylated polypeptides from nonglycosylated polypeptides with which they co-electrophoresed on methylenebisacrylamide cross-linked gels. Purified virions of HSV-1 [F1] had a protein/DNA mass ratio of 10.7 +/- 0.96, and based on a DNA molecular mass of 85 x 10(6) to 100 x 10(6) the estimated weight of virion polypeptides ranges from 16.4 to 19.4 x 10(-16) g. The number of molecules of each polypeptide per virion ranged from less than 50 to 1,500. Comparison of the virion polypeptides of two HSV-1 strains with similar isolation and limited passage history with those of four HSV-1 strains with histories of numerous passages outside the human host showed a number of nonrandom variations in virion polypeptides. Thus, although the virion polypeptides of two strains with similar isolation and limited passage history could not be differentiated, strains with extended passage histories differed markedly from each other and from the limited passage strains in the number and electrophoretic mobility of noncapsid polypeptides and notably in those of the envelope.
单纯疱疹病毒1型（人疱疹病毒1）F1株的纯化病毒粒子中的多肽，在分离后于细胞培养中传代次数有限，在与N，N'-二烯丙基酒石酸二酰胺交联的聚丙烯酰胺凝胶中进行电泳分离时形成了33条带，相比之下，在与N，N'-亚甲基双丙烯酰胺交联的凝胶中最大分辨率仅为24至25条带。条带数量的增加主要是由于糖基化多肽与非糖基化多肽的分离得到改善，它们在亚甲基双丙烯酰胺交联的凝胶上共同电泳。单纯疱疹病毒1型[F1]的纯化病毒粒子的蛋白质/DNA质量比为10.7±0.96，基于DNA分子量85×10（6）至100×10（6），病毒粒子多肽的估计重量范围为16.4至19.4×10（-16）g。每个病毒粒子中每种多肽的分子数范围从少于50到1500。将具有相似分离和有限传代历史的两种单纯疱疹病毒1型菌株的病毒粒子多肽与在人类宿主外有多次传代历史的四种单纯疱疹病毒1型菌株的病毒粒子多肽进行比较，发现病毒粒子多肽存在许多非随机变化。因此，尽管具有相似分离和有限传代历史的两种菌株的病毒粒子多肽无法区分，但传代历史较长的菌株在非衣壳多肽的数量和电泳迁移率上，特别是包膜多肽的数量和电泳迁移率上，彼此之间以及与有限传代菌株有明显差异。
