Kajiyama Hiroaki, Kikkawa Fumitaka, Suzuki Takahiro, Shibata Kiyosumi, Ino Kazuhiko, Mizutani Shigehiko
Department of Obstetrics and Gynecology, Nagoya Graduate University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466-8550, Japan.
Cancer Res. 2002 May 15;62(10):2753-7.
Dipeptidyl peptidase IV (DPPIV) is a multifunctional cell surface aminopeptidase with ubiquitous expression. Recent studies have suggested that DPPIV plays an important role in tumor progression in several human malignancies. In the present study, we investigated the correlation between DPPIV expression and progressive potential in ovarian carcinoma. We demonstrated that ovarian carcinoma cell lines with higher DPPIV expression were less invasive. Furthermore, DPPIV overexpression in SKOV3 cells, derived from serous cystadenocarcinoma, with little DPPIV expression induced a dramatic change in cellular morphology and a significant decrease in the abilities of both migration and invasion. In addition, we have also shown that nude mice inoculated with DPPIV-transfected SKOV3 cells showed significantly less peritoneal dissemination and longer survival time than those receiving the parental or vector-only transfected cells (mean survival time, 64.9 +/- 4.7, 35.7 +/- 2.8, and 36.6 +/- 1.8 days, respectively). This evidence implies that DPPIV may functionally suppress peritoneal dissemination in ovarian carcinoma.
二肽基肽酶IV（DPPIV）是一种多功能细胞表面氨基肽酶，表达广泛。最近的研究表明，DPPIV在几种人类恶性肿瘤的肿瘤进展中起重要作用。在本研究中，我们调查了DPPIV表达与卵巢癌进展潜能之间的相关性。我们证明，DPPIV表达较高的卵巢癌细胞系侵袭性较低。此外，在几乎不表达DPPIV的浆液性囊腺癌来源的SKOV3细胞中过表达DPPIV，会导致细胞形态发生显著变化，并使迁移和侵袭能力显著降低。此外，我们还表明，接种了DPPIV转染的SKOV3细胞的裸鼠比接受亲本细胞或仅载体转染细胞的裸鼠腹膜播散明显减少，存活时间更长（平均存活时间分别为64.9±4.7、35.7±2.8和36.6±1.8天）。这一证据表明，DPPIV可能在功能上抑制卵巢癌的腹膜播散。
